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The human kidney capsule contains a functionally distinct mesenchymal stromal cell population
- Source :
- PLoS ONE, 12(12), PLoS ONE, Vol 12, Iss 12, p e0187118 (2017), PLoS ONE
- Publication Year :
- 2017
-
Abstract
- We recently demonstrated that the adult human kidney cortex contains a perivascular stromal cell (kPSC) that shows organotypic properties and is important for repair and stabilisation of kidney function. Not only the kidney cortex but also the kidney capsule contains stromal cells that are important for the three dimensional organisation of the kidney during nephrogenesis. They provide the barrier function of the capsule which is critical for homeostatic processes such as pressure natriuresis. We postulated that stromal cells derived from the kidney capsule may therefore also have specific properties and functions. To this end, we isolated these capsule mesenchymal stromal cells (cMSC) from human cadaveric kidneys that were not suitable for transplantation. There were several similarities between cMSCs and kPSCs including support of vascular plexus formation, phenotypic marker expression and resistance against myofibroblast transformation. However, compared to kPSCs, cMSCs showed distinct mRNA and miRNA expression profiles, showed increased immunosuppressive capacity, and displayed strongly reduced HGF production, contributing to the inability to enhance kidney epithelial repair. Therefore cMSCs are a distinct, novel human kidney-derived MSC-population and these data underpin the large functional diversity of phenotypic similar stromal cells in relation to their anatomic site, even within one organ.
- Subjects :
- 0301 basic medicine
Physiology
Cellular differentiation
Kidney
Biochemistry
Endocrinology
Animal Cells
Transforming Growth Factor beta
Immune Physiology
Medicine and Health Sciences
Cells, Cultured
Connective Tissue Cells
Innate Immune System
education.field_of_study
Multidisciplinary
Cell Differentiation
Cell biology
Nucleic acids
medicine.anatomical_structure
Connective Tissue
Cytokines
Medicine
Anatomy
Cellular Types
Myofibroblast
Research Article
Stromal cell
Science
Immunology
Population
Excretion
Biology
03 medical and health sciences
Growth Factors
Genetics
medicine
Humans
Cell Lineage
RNA, Messenger
Non-coding RNA
education
Endocrine Physiology
Gene Expression Profiling
Mesenchymal stem cell
Biology and Life Sciences
Kidney metabolism
Kidneys
Mesenchymal Stem Cells
Renal System
Cell Biology
Molecular Development
Coculture Techniques
Gene regulation
Transplantation
MicroRNAs
Biological Tissue
030104 developmental biology
Immune System
RNA
Gene expression
Stromal Cells
Physiological Processes
Developmental Biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, 12(12), PLoS ONE, Vol 12, Iss 12, p e0187118 (2017), PLoS ONE
- Accession number :
- edsair.doi.dedup.....d7f3f2e334aa36923eb67a33fe14f6ad