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Influence of the vitamin D plasma level and vitamin D-related genetic polymorphisms on the immune status of patients with type 1 diabetes: a pilot study
- Source :
- Clinical & Experimental Immunology; Vol 171
- Publication Year :
- 2013
- Publisher :
- WILEY-BLACKWELL, 2013.
-
Abstract
- Summary Vitamin D (VD) has been implicated in type 1 diabetes (T1D) by genetic and epidemiological studies. Individuals living in regions with low sunlight exposure have an increased T1D risk and VD supplementation reduced the risk in human individuals and mouse models. One possibility of how VD influences the pathogenesis of T1D is its immunomodulatory effect on dendritic cells (DC), which then preferentially activate regulatory T cells (Tregs). In the present pilot study, we collected blood samples from a small cohort of patients with T1D at baseline and months 6 and 12. VD-deficient patients were advised to supplement with 1000 IU/day VD. We found a considerable variation in the VD plasma level at baseline and follow-up. However, with higher VD plasma levels, a lower frequency of interleukin (IL)-4-producing CD8 T cells was observed. We further performed a comprehensive genotyping of 13 VD-related polymorphisms and found an association between VD plasma level and the genotype of the VD binding protein (DBP). The frequency of DC and T cell subsets was variable in patients of all subgroups and in individual patients over time. Nevertheless, we found some significant associations, including the 1,25-dihydroxyvitamin D3 hydroxylase (CYP27B1) genotype with the frequency of DC subtypes. In summary, our preliminary results indicate only a limited influence of the VD plasma level on the immune balance in patients with T1D. Nevertheless, our pilot study provides a basis for a follow-up study with a larger cohort of patients.
- Subjects :
- medicine.medical_specialty
T cell
Immunology
DNA Mutational Analysis
030209 endocrinology & metabolism
Pilot Projects
Biology
CD8-Positive T-Lymphocytes
Lymphocyte Activation
T-Lymphocytes, Regulatory
03 medical and health sciences
Mice
0302 clinical medicine
Immune system
Internal medicine
Diabetes mellitus
Vitamin D and neurology
medicine
Immunology and Allergy
Cytotoxic T cell
Animals
Humans
Genetic Predisposition to Disease
Vitamin D
Cells, Cultured
030304 developmental biology
Cell Proliferation
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
0303 health sciences
Type 1 diabetes
Polymorphism, Genetic
Interleukin
Dendritic Cells
Original Articles
Middle Aged
medicine.disease
3. Good health
DNA-Binding Proteins
Endocrinology
medicine.anatomical_structure
Diabetes Mellitus, Type 1
Cohort
Interleukin-4
Follow-Up Studies
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 171
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Clinical & Experimental Immunology
- Accession number :
- edsair.doi.dedup.....d7ec43fdc19ab65d97267806348bf03b
- Full Text :
- https://doi.org/10.1111/cei.12013