Back to Search
Start Over
Redundant type II cadherins define neuroepithelial cell states for cytoarchitectonic robustness
- Source :
- Communications Biology, Vol 3, Iss 1, Pp 1-16 (2020), Communications Biology
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Individual cell shape and integrity must precisely be orchestrated during morphogenesis. Here, we determine function of type II cadherins, Cdh6, Cdh8, and Cdh11, whose expression combinatorially demarcates the mouse neural plate/tube. While CRISPR/Cas9-based single type II cadherin mutants show no obvious phenotype, Cdh6/8 double knockout (DKO) mice develop intermingled forebrain/midbrain compartments as these two cadherins’ expression opposes at the nascent boundary. Cdh6/8/11 triple, Cdh6/8 or Cdh8/11 DKO mice further cause exencephaly just within the cranial region where mutated cadherins’ expression merges. In the Cdh8/11 DKO midbrain, we observe less-constricted apical actin meshwork, ventrally-directed spreading, and occasional hyperproliferation among dorsal neuroepithelial cells as origins for exencephaly. These results provide rigid evidence that, by conferring distinct adhesive codes to each cell, redundant type II cadherins serve essential and shared roles in compartmentalization and neurulation, both of which proceed under the robust control of the number, positioning, constriction, and fluidity of neuroepithelial cells.<br />Hiraga et al. generate knockout mice lacking type II cadherins Cdh6, Cdh8 and Cdh11 to elucidate their role in the developing CNS. Whereas no phenotype is observed in single knockouts, the analysis of double and triple knockouts show that type II cadherins serve essential and shared roles in compartmentalization and neurulation.
- Subjects :
- Embryology
Neural Tube
Morphogenesis
Neuroepithelial Cells
Medicine (miscellaneous)
Embryonic Development
Fluorescent Antibody Technique
Biology
Exencephaly
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Animals
Humans
lcsh:QH301-705.5
Actin
030304 developmental biology
Gene Editing
0303 health sciences
Neural Plate
Cadherin
Chromosome Mapping
Gene Expression Regulation, Developmental
Cell Differentiation
Genomics
medicine.disease
Cadherins
Immunohistochemistry
Cell biology
Neuroepithelial cell
Neurulation
lcsh:Biology (General)
Forebrain
Gene Targeting
CRISPR-Cas Systems
General Agricultural and Biological Sciences
Neural plate
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 23993642
- Volume :
- 3
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Communications Biology
- Accession number :
- edsair.doi.dedup.....d7d9562ab3dc1aa87eb454833c744057