Back to Search
Start Over
NADPH Oxidase-Mediated Reactive Oxygen Species Production: Subcellular Localization and Reassessment of Its Role in Plant Defense
- Source :
- Molecular Plant-Microbe Interactions, Molecular Plant-Microbe Interactions, American Phytopathological Society, 2009, 22 (7), pp.868-881. ⟨10.1094/MPMI-22-7-0868⟩
- Publication Year :
- 2009
- Publisher :
- Scientific Societies, 2009.
-
Abstract
- International audience; Chemiluminescence detection of reactive oxygen species (ROS) triggered in tobacco BY-2 cells by the fungal elicitor cryptogein was previously demonstrated to be abolished in cells transformed with an antisense construct of the plasma membrane NADPH oxidase, NtrbohD. Here, using electron microscopy, it has been confirmed that the first hydrogen peroxide production occurring a few minutes after challenge of tobacco cells with cryptogein is plasma membrane located and NtrbohD mediated. Furthermore, the presence of NtrbohD in detergent-resistant membrane fractions could be associated with the presence of NtrbohD-mediated hydrogen peroxide patches along the plasma membrane. Comparison of the subcellular localization of ROS in wild-type tobacco and in plants transformed with antisense constructs of NtrbohD revealed that this enzyme is also responsible for the hydrogen peroxide production occurring at the plasma membrane after infiltration of tobacco leaves with cryptogein. Finally, the reactivity of wild-type and transformed plants to the elicitor and their resistance against the pathogenic oomycete Phytophthora parasitica were examined. NtrbohD-mediated hydrogen peroxide production does not seem determinant for either hypersensitive response development or the establishment of acquired resistance but it is most likely involved in the signaling pathways associated with the protection of the plant cell.
- Subjects :
- 0106 biological sciences
Physiology
Biology
01 natural sciences
DNA, Antisense
Fungal Proteins
03 medical and health sciences
Microscopy, Electron, Transmission
NtrbohD
Tobacco
Gene expression
NADPH
Plant defense against herbivory
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Cells, Cultured
Plant Proteins
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Reactive oxygen species
Oxidase test
NADPH oxidase
Hydrogen Peroxide
General Medicine
Plants, Genetically Modified
Subcellular localization
Elicitor
Plant Leaves
Enzyme
chemistry
Biochemistry
biology.protein
REACTIVE OXYGEN SPECIES (ROS)
Oxidoreductases
Reactive Oxygen Species
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 19437706 and 08940282
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Molecular Plant-Microbe Interactions®
- Accession number :
- edsair.doi.dedup.....d7cb6e315c979b95ecbb241714cb6f12