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Does blastocyst mitochondrial DNA content affect miscarriage rate in patients undergoing single euploid frozen embryo transfer?
- Source :
- J Assist Reprod Genet
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- PURPOSE: To determine whether the blastocyst mitochondrial DNA (mtDNA) content is related to the miscarriage rate in patients undergoing single euploid frozen embryo transfer (SEFET). METHODS: A total of 355 single euploid frozen embryo transfer cycles were studied retrospectively between April 2017 and December 2018. A trophectoderm biopsy was performed on day 5/6 blastocysts. Post next-generation sequencing (NGS), the mtDNA content was calculated as the ratio of mitochondrial DNA over nuclear DNA, and the association between blastocyst mtDNA content and miscarriage rate was evaluated. RESULT(S): Three hundred fifty-five euploid blastocysts were selected for SEFET in 314 patients with an average age of 33.7 ± 5.6 years; 255 were biopsied on day 5 (71.8%) and 100 on day 6 (28.2%). Frozen embryo transfer (FET) was performed either in a hormone replacement therapy (HRT) cycle (71.8%; n = 255) or in a natural cycle (NC) (28.2%; n = 100). A pregnancy rate of 66.2% (235/355) was obtained with clinical pregnancy and miscarriage rates of 52.4% (n = 186) and 5.6% (n = 20), respectively. There was no significant difference neither between the blastocyst mtDNA content of pregnant and nonpregnant patients (27.7 ± 9.2 vs. 29.4 ± 8.6, P = 0.095) nor between patients with a clinical pregnancy and miscarriage (30.5 ± 9.3 vs. 27.3 ± 9.2, P = 0.136). Multivariate logistic regression analysis showed the same nonsignificant relationship, except for the miscarriage rate and BMI (OR 1.149, 95% CI 1.03–1.28; P = 0.012). CONCLUSION(S): Mitochondrial DNA content is unable to predict the miscarriage of implanted human euploid blastocysts. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10815-020-02050-8.
- Subjects :
- Adult
0301 basic medicine
Mitochondrial DNA
Pregnancy Rate
Embryonic Development
United Arab Emirates
Fertilization in Vitro
DNA, Mitochondrial
Miscarriage
Andrology
Young Adult
03 medical and health sciences
0302 clinical medicine
Pregnancy
Genetics
medicine
Humans
In patient
Embryo Implantation
Blastocyst
Assisted Reproduction Technologies
Genetics (clinical)
Retrospective Studies
Ploidies
030219 obstetrics & reproductive medicine
business.industry
Obstetrics and Gynecology
General Medicine
Middle Aged
Aneuploidy
Embryo Transfer
medicine.disease
Embryo transfer
Abortion, Spontaneous
Pregnancy rate
030104 developmental biology
medicine.anatomical_structure
Reproductive Medicine
embryonic structures
Female
Ploidy
business
Developmental Biology
Trophectoderm biopsy
Subjects
Details
- ISSN :
- 15737330 and 10580468
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Journal of Assisted Reproduction and Genetics
- Accession number :
- edsair.doi.dedup.....d7c12687a25d6b2018a6ab029ba2b3ce