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Establishment of a High-Resolution Liquid Chromatography–Mass Spectrometry Spectral Library for Screening Toxic Natural Products
- Source :
- Journal of Analytical Toxicology. 46:303-321
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- Many natural products have biological effects on humans and animals. Poisoning caused by natural products is common in clinical toxicology cases. Liquid chromatography–high-resolution mass spectrometry (LC–HRMS) has recently emerged as a powerful analytical tool for large-scale target screening, and the application of LC–HRMS can be expanded to evaluate potential natural product poisoning in clinical cases. We report the construction of an LC–HRMS spectral library of 95 natural products commonly implicated in poisoning, and an LC–HRMS assay was validated for definitive detection of natural products in urine and serum samples. For each compound, the limit of detection was determined in the analytical range of 1.0–1,000 ng/mL for urine samples and 0.50–500 ng/mL for serum samples. The mean (SD) values of matrix effects for urine samples and that for serum samples were both −21% (22%), and the mean (SD) value of recovery for serum samples was 89% (26%). The LC–HRMS assay was successfully applied to identify natural products in clinical cases. The spectral library parameters of each compound are provided in the supplementary material to aid other laboratories in identification of unknown natural toxins and development of similar methods on different mass spectrometry platforms.
- Subjects :
- Resolution (mass spectrometry)
Health, Toxicology and Mutagenesis
Urine
Clinical toxicology
Toxicology
Mass spectrometry
01 natural sciences
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Tandem Mass Spectrometry
Liquid chromatography–mass spectrometry
Animals
Environmental Chemistry
030212 general & internal medicine
Detection limit
Biological Products
Chemical Health and Safety
Natural product
Chromatography
010401 analytical chemistry
Serum samples
0104 chemical sciences
chemistry
Biological Assay
Chromatography, Liquid
Subjects
Details
- ISSN :
- 19452403 and 01464760
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Journal of Analytical Toxicology
- Accession number :
- edsair.doi.dedup.....d7c04543618bfa06ab8bcec15f0414b7