The condition for pseudo-first-order kinetics in enzymatic reactions is independent of the initial enzyme concentration

Authors :: Claudio Mendoza
Santiago Schnell
Source :: Biophysical chemistry. 107(2)
Publication Year :: 2003
Abstract: The linearization of the Michaelis-Menten reaction by pseudo-first-order kinetics is revised. A phase-plane analysis allows the derivation of a new condition for its validity that is directly linked to the reaction efficiency, and contrary to widely established knowledge, is independent of the initial enzyme concentration.

Subjects :: chemistry.chemical_classification
Hanes–Woolf plot
Chemistry
Quantitative Biology::Molecular Networks
Organic Chemistry
Kinetics
Biophysics
Thermodynamics
Numerical Analysis, Computer-Assisted
Biochemistry
Michaelis–Menten kinetics
Enzyme catalysis
Enzymes
Quantitative Biology::Subcellular Processes
Reaction rate constant
Enzyme
Linearization
Pseudo first order

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