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MiRNA-10b marks aggressive squamous cell carcinomas, and confers a cancer stem cell-like phenotype

Authors :
Christina Guttmann-Gruber
Mila Sajinovic
Verena Wally
Norbert Niklas
Stefan Hainzl
Johann W. Bauer
Eva M. Murauer
Dirk Strunk
Johannes Proell
Michael Ablinger
Thomas Kocher
Josefina Piñón-Hofbauer
Julia Reichelt
Paul de Souza
Manuela Reisenberger
Albert S. Mellick
Thomas Lettner
R. Zauner
M. Wimmer
Publication Year :
2020
Publisher :
Cold Spring Harbor Laboratory, 2020.

Abstract

BackgroundCutaneous squamous cell carcinomas (cSCC) are the primary cause of premature deaths in patients suffering from the rare skin-fragility disorder recessive dystrophic epidermolysis bullosa, which is in marked contrast to the rarely metastasizing nature of these carcinomas in the general population. This remarkable difference is attributed to the frequent development of chronic wounds caused by an impaired skin integrity. However, the specific molecular and cellular changes to malignancy, and whether there are common players in different types of aggressive cSCCs, remain relatively undefined.MethodsMiRNA expression profiling was performed across various cell types isolated from skin and cSCCs. Microarray results were confirmed by qPCR and by an optimized in situ hybridization protocol. Functional impact of overexpression of a dysregulated miRNA was assessed in migration and 3D spheroid assays. Sample-matched transcriptome data was generated to support the identification of disease relevant miRNA targets.ResultsSeveral miRNAs were identified as dysregulated in cSCCs as compared to controls. These included the metastasis-linked miR-10b, which was significantly upregulated in primary cell cultures and in archival biopsies. At the functional level, overexpression of miR-10b conferred the stem cell-characteristic of 3D-spheroid formation capacity to keratinocytes, and impaired their mobility. Analysis of miR-10b downstream effects identified a novel putative target of miR-10b, the actin- and tubulin cytoskeleton-associated protein DIAPH2.ConclusionThe discovery that miR-10b confers an aspect of cancer stemness – that of enhanced tumor cell adhesion, known to facilitate metastatic colonization - provides an important avenue for future development of novel therapies targeting this metastasis-linked miRNA.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....d79b9b945a245cc77296c1d38b84349e
Full Text :
https://doi.org/10.1101/2020.02.05.934109