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In Vivo Screening Using Transgenic Zebrafish Embryos Reveals New Effects of HDAC Inhibitors Trichostatin A and Valproic Acid on Organogenesis
- Source :
- PLoS ONE, Vol 11, Iss 2, p e0149497 (2016), PLoS ONE, PLoS ONE, Public Library of Science, 2016, 11 (2), pp.e0149497. ⟨10.1371/journal.pone.0149497⟩, PLoS ONE, 2016, 82, pp.23-23. ⟨10.1371/journal.pone.0149497⟩, PLoS ONE, Public Library of Science, 2016, 82, pp.23-23. ⟨10.1371/journal.pone.0149497⟩, PLoS ONE, 2016, 11 (2), pp.e0149497. ⟨10.1371/journal.pone.0149497⟩, Plos One 2 (11), e0149497. (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- International audience; The effects of endocrine disrupting chemicals (EDCs) on reproduction are well known, whereas their developmental effects are much less characterized. However, exposure to endocrine disruptors during organogenesis may lead to deleterious and permanent problems later in life. Zebrafish (Danio rerio) transgenic lines expressing the green fluorescent protein (GFP) in specific organs and tissues are powerful tools to uncover developmental defects elicited by EDCs. Here, we used seven transgenic lines to visualize in vivo whether a series of EDCs and other pharmaceutical compounds can alter organogenesis in zebra-fish. We used transgenic lines expressing GFP in pancreas, liver, blood vessels, inner ear, nervous system, pharyngeal tooth and pectoral fins. This screen revealed that four of the tested chemicals have detectable effects on different organs, which shows that the range of effects elicited by EDCs is wider than anticipated. The endocrine disruptor tetrabromobi-sphenol-A (TBBPA), as well as the three drugs diclofenac, trichostatin A (TSA) and valproic acid (VPA) induced abnormalities in the embryonic vascular system of zebrafish. Moreover, TSA and VPA induced specific alterations during the development of pancreas, an observation that was confirmed by in situ hybridization with specific markers. Developmental delays were also induced by TSA and VPA in the liver and in pharyngeal teeth, resulting in smaller organ size. Our results show that EDCs can induce a large range of developmental alterations during embryogenesis of zebrafish and establish GFP transgenic lines as powerful tools to screen for EDCs effects in vivo.
- Subjects :
- 0301 basic medicine
Embryology
Teeth
Organogenesis
lcsh:Medicine
poisson zèbre
010501 environmental sciences
Hydroxamic Acids
Mechanical Treatment of Specimens
Biochemistry
01 natural sciences
Green fluorescent protein
Animals, Genetically Modified
Fats
Larvae
Medicine and Health Sciences
lcsh:Science
[SDV.BDD]Life Sciences [q-bio]/Development Biology
Zebrafish
Multidisciplinary
biology
Fishes
Animal Models
Chemical Disruption
Lipids
Cell biology
Specimen Disruption
Endocrine disruptor
Osteichthyes
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Vertebrates
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Anatomy
Research Article
medicine.drug
Danio
Endocrine System
In situ hybridization
Research and Analysis Methods
Green Fluorescent Protein
03 medical and health sciences
Model Organisms
Exocrine Glands
In vivo
medicine
Animals
Pancreas
0105 earth and related environmental sciences
inhibiteur
Metamorphosis
Valproic Acid
Embryos
lcsh:R
Organisms
Biology and Life Sciences
Proteins
Lipid Metabolism
biology.organism_classification
Histone Deacetylase Inhibitors
Luminescent Proteins
[SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis
030104 developmental biology
Trichostatin A
Jaw
Specimen Preparation and Treatment
embryon
organogénèse
lcsh:Q
Digestive System
Head
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....d79981a336e750d0aed43411edcef964