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Inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles
- Source :
- Food and Agricultural Immunology, Vol 32, Iss 1, Pp 740-753 (2021)
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- In this study, we present an inner filter effect-based immunoassay (IFE-IA) for the detection of acetamiprid with a turn off–on model. In the IFE-IA, the monoclonal antibody (mAb) conjugated with upconversion nanoparticles (UCNPs) were set as a fluorescence source, and the antigen conjugated with gold nanoparticles (AuNPs) were set as the fluorescence switch off reagent, while acetamiprid acts as the fluorescence turn-on reagent. After optimization, the concentration of acetamiprid producing 50% saturation of the signal (SC50) was 0.04 µg/L, and the detection range (SC10–SC90) was 0.002–0.58 µg/L. The IFE-IA showed no cross-reactivity with the analogues of acetamiprid except for thiacloprid (36.4%). The average recoveries of the immunoassay for spiked samples were in the range of 75.1–104.7%. In addition, the assay showed good correlation with high-performance liquid chromatography in the detection of blind samples.
- Subjects :
- medicine.drug_class
acetamiprid
Agriculture (General)
Immunology
Conjugated system
Monoclonal antibody
Acetamiprid
S1-972
chemistry.chemical_compound
Upconversion nanoparticles
medicine
immunoassay
Chromatography
integumentary system
medicine.diagnostic_test
Chemistry
RC581-607
upconversion nanoparticles
Colloidal gold
gold nanoparticles
Immunoassay
inner filter effect
Filter effect
Immunologic diseases. Allergy
Agronomy and Crop Science
Food Science
Subjects
Details
- ISSN :
- 14653443 and 09540105
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Food and Agricultural Immunology
- Accession number :
- edsair.doi.dedup.....d77ea252a79c5bdddca966d044967bd2