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Fibroblast-associated tumour microenvironment induces vascular structure-networked tumouroid
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
- Publication Year :
- 2017
-
Abstract
- In vitro three-dimensional (3D) tumour models mimic natural cancer tissue in vivo, bridging the gap between conventional 2D in vitro testing and animal models. Stromal and cancer tissues with extracellular matrix (ECM) can provide a tumour microenvironment (TME) with cell-to-cell and cell-to-ECM interactions. These interactions induce the exchange of biophysical factors, contributing to the progression, metastasis, and drug resistance of cancer. Here, we describe a 3D in vitro lung cancer model cultured in a microfluidic channel that is able to confirm the role and function of various stromal cells in tumourigenesis, thereby representing an in vivo-like TME. We founded that biophysical factors contribute to the role of fibroblast cells in tumour formation, especially, producing a nascent vessel-like tubular structure, resulting in the formation of vascularized tumour tissue. Fibroblast cells altered the gene expression of the cancer cells to enhance metastasis, survival, and angiogenesis. The device could be used for developing and screening anti-cancer drugs through the formation of the same multicellular tumour spheroids under TME interactions. We believe this microfluidic system provides interaction of TME for cancer research by culturing stromal tissue.
- Subjects :
- 0301 basic medicine
Stromal cell
Lung Neoplasms
Angiogenesis
Microfluidics
lcsh:Medicine
Neovascularization, Physiologic
Models, Biological
Article
Metastasis
Extracellular matrix
03 medical and health sciences
Organ Culture Techniques
medicine
Tumor Microenvironment
Animals
Fibroblast
lcsh:Science
Cells, Cultured
Tumor microenvironment
Multidisciplinary
Chemistry
lcsh:R
Cancer
Fibroblasts
medicine.disease
030104 developmental biology
medicine.anatomical_structure
Cancer cell
Cancer research
lcsh:Q
Stromal Cells
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....d773bb8c8e8ba81ed977ba5d444af090