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Antimicrobial activity, toxicity and accumulated hard‐tissue debris (AHTD) removal efficacy of several chelating agents
- Source :
- International Endodontic Journal. 53:1093-1110
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Aim: To evaluate the antimicrobial, toxicity and cleaning effectiveness of ethylenediaminetetraacetic acid (EDTA) and maleic acid (MA) alone and combined with cetrimide (CTR). Methodology: Cytotoxic and genotoxic effects were assessed on Chinese hamster cells V79 using the MTT, clonogenic and micronucleus assays, respectively. The bacterial inhibitory and bactericidal concentrations (MIC and MBC, respectively) were determined on a strain of Enterococcus faecalis. Antimicrobial tests were performed on a biofilm model after treatment with the chelating agents by using a biofilm eradication concentration (MBEC) and confocal laser scanning microscope (CLSM) assays. Quantification of cell biomass and percentage of live and dead cells in the biomass were assessed for each group. The percentage reduction of accumulated hard-tissue debris (AHTD) after root canal preparation and final irrigation protocols was evaluated by micro-CT. Statistical tests of one-way analysis of variance (anova), Bonferroni test, Kruskal–Wallis test, Dunn’s multiple comparison test and Wilcoxon matched-pairs signed-rank tests were used. Results: Cetrimide alone as well as in combination with EDTA and MA at dilutions of 1/10 and 1/100 was significantly more toxic as compared to untreated controls (P
- Subjects :
- Micro-CT
Serial dilution
Sodium Hypochlorite
0206 medical engineering
Cetrimide
Ethylenediaminetetraacetic acid
02 engineering and technology
biofilm
irrigation
Enterococcus faecalis
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Anti-Infective Agents
Cricetinae
Animals
Chelation
Food science
Clonogenic assay
General Dentistry
Edetic Acid
Chelating Agents
confocal laser scanning microscopy
Root Canal Irrigants
biology
Chemistry
030206 dentistry
biology.organism_classification
Antimicrobial
020601 biomedical engineering
chelating agent
Toxicity
Dental Pulp Cavity
Root Canal Preparation
Subjects
Details
- ISSN :
- 13652591 and 01432885
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- International Endodontic Journal
- Accession number :
- edsair.doi.dedup.....d7656297e4e8b396aff0e39895d02980