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Evaluation of Allogeneic Bone-Marrow-Derived and Umbilical Cord Blood-Derived Mesenchymal Stem Cells to Prevent the Development of Osteoarthritis in An Equine Model
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, MDPI, 2021, 22 (5), pp.2499. ⟨10.3390/ijms22052499⟩, Volume 22, Issue 5, International Journal of Molecular Sciences, Vol 22, Iss 2499, p 2499 (2021)
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- International audience; Osteoarthritis (OA) is a significant cause of pain in both humans and horses with a high socio-economic impact. The horse is recognized as a pertinent model for human OA. In both species, regenerative therapy with allogeneic mesenchymal stem cells (MSCs) appears to be a promising treatment but, to date, no in vivo studies have attempted to compare the effects of different cell sources on the same individuals. The objective of this study is to evaluate the ability of a single blinded intra-articular injection of allogeneic bone-marrow (BM) derived MSCs and umbilical cord blood (UCB) derived MSC to limit the development of OA-associated pathological changes compared to placebo in a post-traumatic OA model applied to all four fetlock joints of eight horses. The effect of the tissue source (BM vs. UCB) is also assessed on the same individuals. Observations were carried out using clinical, radiographic, ultrasonographic, and magnetic resonance imaging methods as well as biochemical analysis of synovial fluid and postmortem microscopic and macroscopic evaluations of the joints until Week 12. A significant reduction in the progression of OA-associated changes measured with imaging techniques, especially radiography, was observed after injection of bone-marrow derived mesenchymal stem cells (BM-MSCs) compared to contralateral placebo injections. These results indicate that allogeneic BM-MSCs are a promising treatment for OA in horses and reinforce the importance of continuing research to validate these results and find innovative strategies that will optimize the therapeutic potential of these cells. However, they should be considered with caution given the low number of units per group.
- Subjects :
- Male
0301 basic medicine
Pathology
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Osteoarthritis
Umbilical cord
Regenerative medicine
Injections, Intra-Articular
lcsh:Chemistry
0403 veterinary science
Synovial Fluid
lcsh:QH301-705.5
Spectroscopy
allogeneic
04 agricultural and veterinary sciences
General Medicine
Fetal Blood
3. Good health
Computer Science Applications
horse
medicine.anatomical_structure
[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
Female
Fetlock
medicine.medical_specialty
bone marrow
pre-clinical study
040301 veterinary sciences
Mesenchymal Stem Cell Transplantation
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
In vivo
medicine
Animals
Synovial fluid
Horses
Physical and Theoretical Chemistry
Molecular Biology
mesenchymal stem cells
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
business.industry
Organic Chemistry
Mesenchymal stem cell
medicine.disease
Arthritis, Experimental
osteoarthritis
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
umbilical cord blood
Bone marrow
business
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, MDPI, 2021, 22 (5), pp.2499. ⟨10.3390/ijms22052499⟩, Volume 22, Issue 5, International Journal of Molecular Sciences, Vol 22, Iss 2499, p 2499 (2021)
- Accession number :
- edsair.doi.dedup.....d75ee349d2ff9653d761b4b0357a189a
- Full Text :
- https://doi.org/10.3390/ijms22052499⟩