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Evaluation of Allogeneic Bone-Marrow-Derived and Umbilical Cord Blood-Derived Mesenchymal Stem Cells to Prevent the Development of Osteoarthritis in An Equine Model

Authors :
Martine Melin
Thomas Branly
Jean-Marie Denoix
Sandrine Jacquet-Guibon
Daniel-Jean Hartmann
Pascaline Rivory
Magali Demoor
Amandine Schmutz
Fabrice Audigié
Florence Legendre
Philippe Galéra
Lélia Bertoni
Mélanie Desancé
Biomécanique et Pathologie Locomotrice du Cheval (BPLC)
École nationale vétérinaire d'Alfort (ENVA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Biologie, génétique et thérapies ostéoarticulaires et respiratoires (BIOTARGEN)
Université de Caen Normandie (UNICAEN)
Normandie Université (NU)-Normandie Université (NU)
NOVOTEC
European Commission2897/33535917RB148917CB174Normandy County Council2013-AGRI-236/13P07492917CB166Fonds EperonN80-2014917CB194GIS CENTAURE equine research (EQUISTEM-G program)014CJ061French National Research Agency (ANR)917RB020917RB072French Ministry of Research and TechnologyCENTAURE European projectEuropean Commission
Source :
International Journal of Molecular Sciences, International Journal of Molecular Sciences, MDPI, 2021, 22 (5), pp.2499. ⟨10.3390/ijms22052499⟩, Volume 22, Issue 5, International Journal of Molecular Sciences, Vol 22, Iss 2499, p 2499 (2021)
Publication Year :
2021
Publisher :
HAL CCSD, 2021.

Abstract

International audience; Osteoarthritis (OA) is a significant cause of pain in both humans and horses with a high socio-economic impact. The horse is recognized as a pertinent model for human OA. In both species, regenerative therapy with allogeneic mesenchymal stem cells (MSCs) appears to be a promising treatment but, to date, no in vivo studies have attempted to compare the effects of different cell sources on the same individuals. The objective of this study is to evaluate the ability of a single blinded intra-articular injection of allogeneic bone-marrow (BM) derived MSCs and umbilical cord blood (UCB) derived MSC to limit the development of OA-associated pathological changes compared to placebo in a post-traumatic OA model applied to all four fetlock joints of eight horses. The effect of the tissue source (BM vs. UCB) is also assessed on the same individuals. Observations were carried out using clinical, radiographic, ultrasonographic, and magnetic resonance imaging methods as well as biochemical analysis of synovial fluid and postmortem microscopic and macroscopic evaluations of the joints until Week 12. A significant reduction in the progression of OA-associated changes measured with imaging techniques, especially radiography, was observed after injection of bone-marrow derived mesenchymal stem cells (BM-MSCs) compared to contralateral placebo injections. These results indicate that allogeneic BM-MSCs are a promising treatment for OA in horses and reinforce the importance of continuing research to validate these results and find innovative strategies that will optimize the therapeutic potential of these cells. However, they should be considered with caution given the low number of units per group.

Details

Language :
English
ISSN :
16616596 and 14220067
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences, International Journal of Molecular Sciences, MDPI, 2021, 22 (5), pp.2499. ⟨10.3390/ijms22052499⟩, Volume 22, Issue 5, International Journal of Molecular Sciences, Vol 22, Iss 2499, p 2499 (2021)
Accession number :
edsair.doi.dedup.....d75ee349d2ff9653d761b4b0357a189a
Full Text :
https://doi.org/10.3390/ijms22052499⟩