Back to Search
Start Over
Threading the barrel of the RNA exosome
- Source :
- Trends in Biochemical Sciences, Schneider, C & Tollervey, D 2013, ' Threading the barrel of the RNA exosome ', Trends in biochemical sciences, vol. 38, no. 10, pp. 485-493 . https://doi.org/10.1016/j.tibs.2013.06.013
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Highlights • A wide range of in vivo targets for the exosome complex has been established. • RNA polymerase III transcripts have emerged as major substrates. • The human nucleus has spatially localized forms of the exosome, with matching cofactors. • Structural analyses reveal a highly conserved RNA path through the eukaryotic exosome.<br />In eukaryotes, the exosome complex degrades RNA backbones and plays key roles in RNA processing and surveillance. It was predicted that RNA substrates are threaded through a central channel. This pathway is conserved between eukaryotic and archaeal complexes, even though nuclease activity was lost from the nine-subunit eukaryotic core (EXO-9) and transferred to associated proteins. The exosome cooperates with nuclear and cytoplasmic cofactors, including RNA helicases Mtr4 and Ski2, respectively. Structures of an RNA-bound exosome and both helicases revealed how substrates are channeled through EXO-9 to the associated nuclease Rrp44. Recent high-throughput analyses provided fresh insights relating exosome structure to its diverse in vivo functions. They also revealed surprisingly high degradation rates for newly synthesized RNAs, particularly RNA polymerase III transcripts.
- Subjects :
- RNA surveillance
Polyadenylation
Exosome complex
RNA-dependent RNA polymerase
Review
Saccharomyces cerevisiae
Biology
Biochemistry
03 medical and health sciences
0302 clinical medicine
exosome
Humans
Molecular Biology
030304 developmental biology
0303 health sciences
RNA quality control
Exosome Multienzyme Ribonuclease Complex
RNA
Molecular biology
Cell biology
RNA silencing
RNA processing
TRAMP complex
exosome cofactor
030217 neurology & neurosurgery
Small nuclear RNA
Subjects
Details
- ISSN :
- 09680004
- Volume :
- 38
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Trends in Biochemical Sciences
- Accession number :
- edsair.doi.dedup.....d7490802c6261d05dc172025a933dc66
- Full Text :
- https://doi.org/10.1016/j.tibs.2013.06.013