Back to Search
Start Over
Incidence and Characterization of Listeria spp. from Foods Available in Korea
- Source :
- Journal of Food Protection. 64:554-558
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- A total of 410 domestic Korean food samples were analyzed for the presence of Listeria spp. by the conventional U.S. Department of Agriculture protocol, and presumptive strains were identified by morphological, cultural and biochemical tests according to Bergey's manual and confirmed by API-Listeria kit. Among the total 410 food samples, 46 samples (11.2%) were found to be contaminated with Listeria species. Among the 46 strains of Listeria spp. isolates, 8 strains (17.42%) for Listeria monocytogenes, 3 strains (6.5%) for Listeria seeligeri, 33 strains (71.7%) for Listeria innocua, and 2 strains (4.4%) for Listeria welshimeri were identified, respectively. Also, only beef, chicken, pork, frozen foods, and sausage were contaminated with L. monocytogenes, and the other products were free of L. monocytogenes. Of 46 Listeria spp. isolates, L. innocua (71.7%) was the most predominantly isolated in a variety of foods compared to other Listeria spp. An in vitro virulence assay for Listeria spp. using myeloma and hybridoma cells from murine and human sources was performed. The result showed that only L. monocytogenes killed approximately 95 to 100% hybridoma cells after 6 h and the other Listeria species, such as L. innocua, L. seeligeri, and L. welshimeri strains had about 0 to 10% lethal effect on hybridoma cells. Also, an antibiotic susceptibility test showed that Listeria spp. isolates were very susceptible to the antibiotics tested, except for nalidixic acid. Also, serotyping results showed 75% of L. monocytogenes isolates from beef, chicken, and frozen pizza belonged to serotype 1 and 25% from sausage were type 4.
- Subjects :
- Serotype
Korea
Meat
Time Factors
Virulence
Nalidixic acid
Listeria
Microbial Sensitivity Tests
Biology
biology.organism_classification
medicine.disease_cause
Microbiology
Listeria welshimeri
Listeria monocytogenes
Food Microbiology
medicine
Animals
Food microbiology
Listeria seeligeri
Serotyping
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 0362028X
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Journal of Food Protection
- Accession number :
- edsair.doi.dedup.....d74832ced900b004690299f1df53a8d8
- Full Text :
- https://doi.org/10.4315/0362-028x-64.4.554