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Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells
- Source :
- Evidence-Based Complementary and Alternative Medicine, Vol 2015 (2015), Evidence-based Complementary and Alternative Medicine : eCAM
- Publication Year :
- 2015
- Publisher :
- Hindawi Limited, 2015.
-
Abstract
- We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro. After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ. Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells. The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P< 0.001); however, QJJ restored HepG2 cell survival (P< 0.001). As compared with HepG2 cells in the IR group, QJJ at all doses analyzed significantly increased glucose consumption (allP< 0.05). Moreover, treatment with all the QJJ doses significantly reduced the mean intracellular reactive oxygen species levels as compared with the IR group (allP< 0.05). Furthermore, high-dose QJJ reduced both TNF-αand IL-6 levels as compared to the IR group (allP< 0.05). QJJ ameliorated the altered PI3K, GLUT4, and RAGE expression observed with IR. In conclusion, QJJ can improve IR in HepG2 cells, which may be mediated through the IRS-1/PI3K/GLUT4 signaling pathway as well as regulation of NF-κB-mediated inflammation and oxidative stress.
- Subjects :
- medicine.medical_specialty
Article Subject
Inflammation
medicine.disease_cause
Palmitic acid
chemistry.chemical_compound
Insulin resistance
Internal medicine
medicine
PI3K/AKT/mTOR pathway
biology
business.industry
lcsh:Other systems of medicine
medicine.disease
lcsh:RZ201-999
Insulin receptor
Endocrinology
Complementary and alternative medicine
chemistry
Immunology
biology.protein
Cytokine secretion
medicine.symptom
business
GLUT4
Oxidative stress
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 17414288
- Volume :
- 2015
- Database :
- OpenAIRE
- Journal :
- Evidence-Based Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....d745e0b5b174a76756a7d5bee925d06e