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Genetic basis for the establishment of endosymbiosis in Paramecium
- Source :
- The ISME Journal
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- The single-celled ciliate Paramecium bursaria is an indispensable model for investigating endosymbiosis between protists and green-algal symbionts. To elucidate the mechanism of this type of endosymbiosis, we combined PacBio and Illumina sequencing to assemble a high-quality and near-complete macronuclear genome of P. bursaria. The genomic characteristics and phylogenetic analyses indicate that P. bursaria is the basal clade of the Paramecium genus. Through comparative genomic analyses with its close relatives, we found that P. bursaria encodes more genes related to nitrogen metabolism and mineral absorption, but encodes fewer genes involved in oxygen binding and N-glycan biosynthesis. A comparison of the transcriptomic profiles between P. bursaria with and without endosymbiotic Chlorella showed differential expression of a wide range of metabolic genes. We selected 32 most differentially expressed genes to perform RNA interference experiment in P. bursaria, and found that P. bursaria can regulate the abundance of their symbionts through glutamine supply. This study provides novel insights into Paramecium evolution and will extend our knowledge of the molecular mechanism for the induction of endosymbiosis between P. bursaria and green algae.
- Subjects :
- Genetics
0303 health sciences
Paramecium
Endosymbiosis
biology
030306 microbiology
Genomics
Chlorella
Bursaria
Brief Communication
biology.organism_classification
Microbiology
Genome
03 medical and health sciences
Paramecium bursaria
Sequencing
Ciliophora
Symbiosis
Gene
Phylogeny
Ecology, Evolution, Behavior and Systematics
Oxygen binding
Illumina dye sequencing
030304 developmental biology
Subjects
Details
- ISSN :
- 17517370 and 17517362
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- The ISME Journal
- Accession number :
- edsair.doi.dedup.....d72abadad95e9c1b55f817a0dc940ccf