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Isolation and Characterization of Polymorphic Microsatellite Loci in Spondias radlkoferi (Anacardiaceae)
- Source :
- Applications in Plant Sciences, Vol 2, Iss 11, p 1400079 (2014)
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- Premise of the study: Microsatellite markers were developed for Spondias radlkoferi to assess the impact of primate seed dispersal on the genetic diversity and structure of this important tree species of Anacardiaceae. Methods and Results: Fourteen polymorphic loci were isolated from S. radlkoferi through 454 GS-FLX Titanium pyrosequencing of genomic DNA. The number of alleles ranged from three to 12. The observed and expected heterozygosities ranged from 0.382 to 1.00 and from 0.353 to 0.733, respectively. The amplification was also successful in S. mombin and two genera of Anacardiaceae: Rhus aromatica and Toxicodendron radicans. Conclusions: These microsatellite loci will be useful to assess the genetic diversity and population structure of S. radlkoferi and related species, and will allow us to investigate the effects of seed dispersal by spider monkeys (Ateles geoffroyi) on the genetic structure and diversity of S. radlkoferi populations in a fragmented rainforest.
- Subjects :
- Genetic diversity
Spondias radlkoferi
biology
Anacardiaceae
Rhus
Seed dispersal
Plant Science
Spondias
Toxicodendron
biology.organism_classification
microsatellites
lcsh:QK1-989
lcsh:Biology (General)
Evolutionary biology
lcsh:Botany
Botany
Genetic structure
Microsatellite
lcsh:QH301-705.5
Rhus aromatica
Ecology, Evolution, Behavior and Systematics
Subjects
Details
- ISSN :
- 21680450
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Applications in Plant Sciences
- Accession number :
- edsair.doi.dedup.....d6ff855cf58c2023eb90e75c4d6636e1