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The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development
- Source :
- EMBO Reports, 19 (3), EMBO Reports
- Publication Year :
- 2018
- Publisher :
- EMBO Press, 2018.
-
Abstract
- MicroRNAs are important regulators of local protein synthesis during neuronal development. We investigated the dynamic regulation of microRNA production and found that the majority of the microRNA‐generating complex, consisting of Dicer, TRBP, and PACT, specifically associates with intracellular membranes in developing neurons. Stimulation with brain‐derived neurotrophic factor (BDNF), which promotes dendritogenesis, caused the redistribution of TRBP from the endoplasmic reticulum into the cytoplasm, and its dissociation from Dicer, in a Ca2+‐dependent manner. As a result, the processing of a subset of neuronal precursor microRNAs, among them the dendritically localized pre‐miR16, was impaired. Decreased production of miR‐16‐5p, which targeted the BDNF mRNA itself, was rescued by expression of a membrane‐targeted TRBP. Moreover, miR‐16‐5p or membrane‐targeted TRBP expression blocked BDNF‐induced dendritogenesis, demonstrating the importance of neuronal TRBP dynamics for activity‐dependent neuronal development. We propose that neurons employ specialized mechanisms to modulate local gene expression in dendrites, via the dynamic regulation of microRNA biogenesis factors at intracellular membranes of the endoplasmic reticulum, which in turn is crucial for neuronal dendrite complexity and therefore neuronal circuit formation and function.<br />EMBO Reports, 19 (3)<br />ISSN:1469-221X<br />ISSN:1469-3178
- Subjects :
- 0301 basic medicine
Ribonuclease III
pre-miRNA processing
Endoplasmic Reticulum
Biochemistry
DEAD-box RNA Helicases
Neurotrophic factors
isomiR
Gene expression
News & Views
Membrane & Intracellular Transport
miR-16
Neurons
biology
Chemistry
RNA-Binding Proteins
Articles
RNA Biology
Cell biology
medicine.anatomical_structure
miR‐16
Intracellular
Neurogenesis
Nuclear Receptor Coactivators
Embryonic Development
Dendrite
Article
03 medical and health sciences
BDNF-induced dendritogenesis
microRNA
Genetics
medicine
Animals
Humans
Molecular Biology
pre‐miRNA processing
Messenger RNA
Endoplasmic reticulum
Brain-Derived Neurotrophic Factor
Dendrites
endoplasmic reticulum
Embryo, Mammalian
Rats
MicroRNAs
030104 developmental biology
nervous system
biology.protein
BDNF‐induced dendritogenesis
Neuroscience
Dicer
Subjects
Details
- Language :
- English
- ISSN :
- 1469221X and 14693178
- Database :
- OpenAIRE
- Journal :
- EMBO Reports, 19 (3), EMBO Reports
- Accession number :
- edsair.doi.dedup.....d6e1328e39c56c4d9a7cc53ed5034688