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Circadian rhythmicity: A functional connection between differentiated embryonic chondrocyte-1 (DEC1) and small heterodimer partner (SHP)
- Source :
- Archives of Biochemistry and Biophysics. 631:11-18
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Circadian rhythm misalignment has been increasingly recognized to pose health risk for a wide range of diseases, particularly metabolic disorders. The liver maintains metabolic homeostasis and expresses many circadian genes, such as differentiated embryo chondrocyte-1 (DEC1) and small heterodimer partner (SHP). DEC1 is established to repress transcription through E-box elements, and SHP belongs to the superfamily of nuclear receptors and has multiple E-box elements in its promoter. Importantly, DEC1 and SHP are inversely oscillated. This study was performed to test the hypothesis that the SHP gene is a target gene of DEC1. Cotransfection demonstrated that DEC1 repressed the SHP promoter and attenuated the transactivation of the classic circadian activator complex of Clock/Bmal1. Site-directed mutagenesis, electrophoretic mobility shift assay and chromatin immunoprecipitation established that the repression was achieved through the E-box in the proximal promoter. Transfection of DEC1 suppressed the expression of SHP. In circadian-inducing cells, the epileptic agent valproate inversely altered the expression of DEC1 and SHP. Both DEC1 and SHP are involved in energy balance and valproate is known to induce hepatic steatosis. Our findings collectively establish that DEC1 participates in the negative loop of SHP oscillating expression with potential implications in metabolic homeostasis.
- Subjects :
- 0301 basic medicine
animal structures
Biophysics
Receptors, Cytoplasmic and Nuclear
chemical and pharmacologic phenomena
Biology
Transfection
Biochemistry
Article
03 medical and health sciences
Transactivation
Basic Helix-Loop-Helix Transcription Factors
Humans
Electrophoretic mobility shift assay
Promoter Regions, Genetic
Molecular Biology
Psychological repression
Homeodomain Proteins
Activator (genetics)
hemic and immune systems
Hep G2 Cells
Circadian Rhythm
DEC1
030104 developmental biology
Gene Expression Regulation
Nuclear receptor
embryonic structures
Small heterodimer partner
Cancer research
biological phenomena, cell phenomena, and immunity
Chromatin immunoprecipitation
Subjects
Details
- ISSN :
- 00039861
- Volume :
- 631
- Database :
- OpenAIRE
- Journal :
- Archives of Biochemistry and Biophysics
- Accession number :
- edsair.doi.dedup.....d6e087707b9d0b0e57e7758a83680c6f