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Opsonin-independent adherence and intracellular development of Legionella pneumophila within U-937 cells
- Source :
- Canadian Journal of Microbiology. 39:718-722
- Publication Year :
- 1993
- Publisher :
- Canadian Science Publishing, 1993.
-
Abstract
- Legionella pneumophila adhered to and multiplied intracellularly in the human histiocytic lymphoma U-937 cell line. The infectious process was evaluated by viable bacterial cell colony counts and documented by transmission and scanning electron microscopy. In the absence of opsonins, wash-resistant bacterial adherence to host cells occurred within 1 h and attachment of 1 or 2 organisms per U-937 host cell involved close surface interactions at the prokaryotic and eukaryotic membranes. Intracellular multiplication of bacteria was maximal by 24 h after inoculation of cell monolayers. Release of L. pneumophila from these cells appeared as a lytic process that resulted in an increase in the numbers of microorganisms in the extracellular fluids and a concomitant decline in the number of intracellular bacteria. The course of cellular infection was completed by 72 h. The cellular and ultrastructural events of L. pneumophila adherence and uptake by U-937 cells in the absence of antibody or complement have been defined. In addition, this work further establishes the U-937 cell as a suitable model for investigating Legionella – host cell interactions.Key words: Legionella, U-937 cells, intracellular replication, opsonin-independent uptake.
- Subjects :
- Immunology
Cell
Colony Count, Microbial
Applied Microbiology and Biotechnology
Microbiology
Legionella pneumophila
Bacterial Adhesion
Bacterial cell structure
Tumor Cells, Cultured
Genetics
medicine
Humans
Molecular Biology
Opsonin
biology
Intracellular parasite
General Medicine
Opsonin Proteins
biology.organism_classification
medicine.anatomical_structure
Lytic cycle
Lymphoma, Large B-Cell, Diffuse
Bacteria
Intracellular
Subjects
Details
- ISSN :
- 14803275 and 00084166
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....d6c0797bf53f5544cf64a740a1b18d34