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Impact of obesity and leptin treatment on adipocyte gene expression in Psammomys obesus
- Source :
- Scopus-Elsevier
-
Abstract
- We examined the effects of leptin treatment on the expression of key genes in adipocyte metabolism in Psammomys obesus (P. obesus), a polygenic rodent model of obesity. Lean and obese P. obesus were given three daily intraperitoneal injections of either saline or leptin (total of 45 mg/kg per day) for 7 days. In lean animals, leptin treatment led to reductions in food intake, body weight and fat mass. Pair-fed animals matched for the reduction in food intake of the lean leptin-treated animals demonstrated similar reductions in body weight and fat mass. In obese P. obesus, leptin treatment failed to have any effect on body weight or body fat mass, indicating leptin resistance. Lipoprotein lipase, hormone-sensitive lipase and peroxisome proliferator activated receptor gamma 2 mRNA levels were significantly reduced in lean leptin-treated animals, whereas pair-fed animals were similar to lean controls. Uncoupling protein 2 and glycerol phosphate acyltransferase were also reduced in the lean leptin-treated animals, but not significantly so. Obese animals did not show any gene expression changes after leptin treatment. In conclusion, high circulating concentrations of leptin in lean P. obesus resulted in decreased gene expression of a number of key lipid enzymes, independent of changes in food intake, body weight and fat mass. These effects of leptin were not found in obese P. obesus.
- Subjects :
- Leptin
Male
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Peroxisome proliferator-activated receptor
Gene Expression
Receptors, Cytoplasmic and Nuclear
Biology
Peptide hormone
chemistry.chemical_compound
Eating
Endocrinology
Internal medicine
Adipocyte
medicine
Adipocytes
Animals
Obesity
chemistry.chemical_classification
Lipoprotein lipase
digestive, oral, and skin physiology
Metabolic disorder
Body Weight
Lipase
medicine.disease
biology.organism_classification
Lipoprotein Lipase
chemistry
Body Composition
Female
Psammomys
Gerbillinae
hormones, hormone substitutes, and hormone antagonists
Transcription Factors
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....d6bb427daa2adb2813bccc5324fd2362