Back to Search
Start Over
A Custom-Made Device for Reproducibly Depositing Pre-metered Doses of Nebulized Drugs on Pulmonary Cells in vitro
- Source :
- Frontiers in bioengineering and biotechnology, Switzerland, Frontiers in Bioengineering and Biotechnology, Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- The deposition of pre-metered doses (i.e., defined before and not after exposition) at the air–liquid interface of viable pulmonary epithelial cells remains an important but challenging task for developing aerosol medicines. While some devices allow quantification of the deposited dose after or during the experiment, e.g., gravimetrically, there is still no generally accepted way to deposit small pre-metered doses of aerosolized drugs or pharmaceutical formulations, e.g., nanomedicines. Here, we describe a straightforward custom-made device, allowing connection to commercially available nebulizers with standard cell culture plates. Designed to tightly fit into the approximately 12-mm opening of either a 12-well Transwell® insert or a single 24-well plate, a defined dose of an aerosolized liquid can be directly deposited precisely and reproducibly (4.8% deviation) at the air–liquid interface (ALI) of pulmonary cell cultures. The deposited dose can be controlled by the volume of the nebulized solution, which may vary in a range from 20 to 200 μl. The entire nebulization-deposition maneuver is completed after 30 s and is spatially homogenous. After phosphate-buffered saline (PBS) deposition, the viability and barrier properties transepithelial electrical resistance (TEER) of human bronchial epithelial Calu-3 cells were not negatively affected. Straightforward in manufacture and use, the device enables reproducible deposition of metered doses of aerosolized drugs to study the interactions with pulmonary cell cultures grown at ALI conditions.
- Subjects :
- 0301 basic medicine
Culture plates
inhalation
Histology
Air liquid interface
Inhalation
aerosol
Chemistry
030111 toxicology
Biomedical Engineering
Bioengineering and Biotechnology
Bioengineering
02 engineering and technology
021001 nanoscience & nanotechnology
epithelial cells
air–liquid interface
In vitro
03 medical and health sciences
pulmonary drug delivery
0210 nano-technology
TP248.13-248.65
Aerosolization
Original Research
Biotechnology
Biomedical engineering
Subjects
Details
- ISSN :
- 22964185
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Frontiers in Bioengineering and Biotechnology
- Accession number :
- edsair.doi.dedup.....d6ba7964820926885c4b826ae7741d85