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Inhibitory Effect of Tangeretin and Cardamonin on Human Intestinal SGLT1 Activity In Vitro and Blood Glucose Levels in Mice In Vivo
- Source :
- Nutrients, Vol 13, Iss 3382, p 3382 (2021), Nutrients, Volume 13, Issue 10
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Rapid postprandial blood glucose elevation can cause lifestyle-related diseases, such as type II diabetes. The absorption of food-derived glucose is primarily mediated by sodium/glucose cotransporter 1 (SGLT1). Moderate SGLT1 inhibition can help attenuate postprandial blood glucose elevation and prevent lifestyle-related diseases. In this study, we established a CHO cell line stably expressing human SGLT1 and examined the effects of phytochemicals on SGLT1 activity. Among the 50 phytochemicals assessed, tangeretin and cardamonin inhibited SGLT1 activity. Tangeretin and cardamonin did not affect the uptake of L-leucine, L-glutamate, and glycyl-sarcosine. Tangeretin, but not cardamonin, inhibited fructose uptake, suggesting that the inhibitory effect of tangeretin was specific to the monosaccharide transporter, whereas that of cardamonin was specific to SGLT1. Kinetic analysis suggested that the suppression of SGLT1 activity by tangeretin was associated with a reduction in Vmax and an increase in Km, whereas suppression by cardamonin was associated with a reduction in Vmax and no change in Km. Oral glucose tolerance tests in mice showed that tangeretin and cardamonin significantly suppressed the rapid increase in blood glucose levels. In conclusion, tangeretin and cardamonin were shown to inhibit SGLT1 activity in vitro and lower blood glucose level in vivo.
- Subjects :
- Blood Glucose
Sodium
Phytochemicals
Administration, Oral
chemistry.chemical_element
CHO Cells
Fructose
Pharmacology
SGLT1
Article
Tangeretin
Chalcones
Cricetulus
Sodium-Glucose Transporter 1
tangeretin
In vivo
Animals
Humans
Monosaccharide
TX341-641
Amino Acids
chemistry.chemical_classification
Mice, Inbred ICR
Nutrition and Dietetics
Chemistry
Nutrition. Foods and food supply
digestive, oral, and skin physiology
Sarcosine
Transporter
intestinal epithelial cell
Flavones
In vitro
Intestines
Kinetics
Postprandial
transporter
cardamonin
Caco-2 Cells
Cotransporter
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Volume :
- 13
- Issue :
- 3382
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....d6b6f819408804282c6617915e0f298c