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Molecular Cloning and Expression Analysis of CD82 in Pig
- Source :
- Animal Biotechnology. 19:237-242
- Publication Year :
- 2008
- Publisher :
- Informa UK Limited, 2008.
-
Abstract
- CD82, which was originally referred to as KAI1 (kangai 1), is a member of the tetraspanin protein family, which contains four transmembrane domains. CD82 is implicated in a variety of biological processes, including apoptosis, cell adhesion, and cell migration. In this study, the full-length cDNA of pig CD82 was cloned and sequenced. Pig Cd82 cDNA contains an open reading frame (801 bp) encoding 266 amino acids. Sequence alignment results indicated that pig CD82 cDNA evidenced 85.45%, 85.63%, 77.03%, and 77.78% identity with human, cattle, rat, and mouse, respectively. In the expression study, the constitutive expression of swine Cd82 mRNA was detected in a variety of tissues, including lymphoid tissues as well as nonlymphoid tissues. Future studies will be focused on the functional role of CD82 during the course of pig infectious diseases or tumor development.
- Subjects :
- Cloning
DNA, Complementary
Base Sequence
Protein family
Reverse Transcriptase Polymerase Chain Reaction
Swine
Molecular Sequence Data
Bioengineering
Sequence alignment
Biology
Molecular cloning
Kangai-1 Protein
Molecular biology
Open reading frame
Transmembrane domain
Complementary DNA
Animals
Female
Animal Science and Zoology
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
Sequence Alignment
Peptide sequence
Biotechnology
Subjects
Details
- ISSN :
- 15322378 and 10495398
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Animal Biotechnology
- Accession number :
- edsair.doi.dedup.....d6b02fba26e4655091adc3ab0884477a