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Presence of platelet-activating factor in rhesus (Macaca mulatta) spermatozoa
- Source :
- Journal of Medical Primatology. 28:32-35
- Publication Year :
- 1999
- Publisher :
- Wiley, 1999.
-
Abstract
- Platelet-activating factor [1-O-alkyl-2-acetyl-sn-glycero-phosphocholine; PAF] is a unique signaling phospholipid which has been implicated in a number of biological activities (e.g., reproduction). PAF has been detected in the spermatozoa from a number of laboratory and domestic species, including, but not limited to, rabbit, bovine, and the mouse. The concentration of PAF is inversely related to human (Homo sapien) spermatozoal quality. Additionally, PAF levels are significantly higher in Bolivian squirrel monkey (Saimiri sciureus) spermatozoa obtained during the breeding season than spermatozoa obtained during the nonbreeding season. There are no reports on the presence of PAF in rhesus (Macaca mulatta) spermatozoa. Therefore, the primary objective of this study was to detect the presence of PAF in rhesus spermatozoa. A second objective was to determine if PAF spermatozoa levels differ between animals housed individually (single-caged) versus free-ranging (open corrals). Semen were collected from mature rhesus via electro-ejaculation. Spermatozoa were washed free of ejaculatory plug and quick frozen in PBS. Endogenous lipids were extracted from thawed spermatozoa and ejaculatory plugs then assayed for the presence of PAF by [125I]-radioimmunoassay. PAF was not detected in any ejaculatory plugs. PAF levels were significantly higher (P < 0.01) in spermatozoa obtained from free-ranging males (mean: 1.16 pmol/10(6) spermatozoa) than males housed individually in single cage units (mean: 0.53 pmol/10(6) spermatozoa). PAF was present in rhesus spermatozoa. Additionally, PAF levels were higher in spermatozoa obtained from corral-housed animals. Additional studies are warranted to elucidate the role of PAF in spermatozoa function.
- Subjects :
- Male
endocrine system
media_common.quotation_subject
Phospholipid
Platelet activating factor metabolism
Semen
Endogeny
Biology
Andrology
Mice
chemistry.chemical_compound
Species Specificity
Stress, Physiological
Animals
Humans
Platelet Activating Factor
reproductive and urinary physiology
media_common
General Veterinary
Platelet-activating factor
urogenital system
Squirrel monkey
respiratory system
biology.organism_classification
Macaca mulatta
Spermatozoa
Rhesus macaque
chemistry
Immunology
Cattle
lipids (amino acids, peptides, and proteins)
Animal Science and Zoology
Rabbits
Reproduction
Subjects
Details
- ISSN :
- 00472565
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Primatology
- Accession number :
- edsair.doi.dedup.....d68de3060e3dfdde9443fe4d9b87652c
- Full Text :
- https://doi.org/10.1111/j.1600-0684.1999.tb00086.x