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Generation of two induced pluripotent stem cell lines (UQACi002-A and UQACi005-A) from two patients with KRT14 epidermolysis bullosa simplex mutations

Authors :
Mbarka Bchetnia
Laurie Martineau
Véronique Racine
Julie Powell
Catherine McCuaig
Charles Morin
Audrey Dupérée
François Gros-Louis
Catherine Laprise
Source :
Stem cell research. 61
Publication Year :
2021

Abstract

More than 107 pathogenic variations were identified in Keratin 14 gene (KRT14) in patients affected by epidermolysis bullosa simplex (EBS), a rare skin disease with still no curative treatment. Disease models as human induced pluripotent stem cells (hiPSCs) are promising tool for further advance the knowledge about this disorder and accelerate therapies development. Here, two hiPSC lines were reprogrammed from skin fibroblasts of two EBS patients carrying mutations within KRT14 by using CytoTune®Sendai virus. These iPSCs display pluripotent cell morphology, pluripotent markers expression, and the capability to differentiate into the three germ layers.

Details

ISSN :
18767753
Volume :
61
Database :
OpenAIRE
Journal :
Stem cell research
Accession number :
edsair.doi.dedup.....d680a8e09d7448449f9f5f7fb4ab9894