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Relationship between adenine nucleotide metabolism and irreversible ischemic tissue damage in isolated perfused rat heart
- Source :
- Circulation research. 45(2)
- Publication Year :
- 1979
-
Abstract
- The relationship between energy metabolism and the extent of irreversible ischemic damage was examined in an isolated perfused working rat heart. The amount of cardiac work recovered after reperfusion of hearts exposed to severe global ischemia was dependent upon both the duration of ischemia and the type of substrate provided (either 5 mM glucose or 5 mM glucose + acetate). There appear to be two distinct phases in the ability to recover mechanical function in the reperfused ischemic heart. The second phase corresponds to the onset of severe irreversible tissue damage. Irreversible mitochondrial damage was not found to correspond with the onset of heart failure since the ATP/ADP ratio remained constant in the reperfused myocardium. Furthermore, there does not appear to be a direct correlation between the total ATP content and the extent of irreversible damage, either during ischemia or following reperfusion. However, the total adenine nucleotide content during ischemia showed dramatic changes which correspond temporally with the initiation of the second phase of damage. The observation that the adenine nucleotide pool becomes further depleted during reperfusion suggests that alterations in the salvage pathway for adenine nucleotide synthesis have occurred. Loss of adenine nucleotides appears to be an excellent marker for irreversible heart failure. Acetate provides some protection the the ischemic myocardium. The mechanism by which acetate mediates this protective effect is discussed.
- Subjects :
- Male
medicine.medical_specialty
Phosphocreatine
Physiology
Ischemia
Coronary Disease
Mitochondria, Heart
Adenosine Triphosphate
Adenine nucleotide
Internal medicine
Coronary Circulation
medicine
Animals
Insulin
Cardiac Output
Nucleotide salvage
Chemistry
Adenine Nucleotides
Myocardium
Substrate (chemistry)
Metabolism
Rat heart
medicine.disease
Rats
Adenosine Diphosphate
Endocrinology
Glucose
Tissue ischemia
Biochemistry
Heart failure
Cardiology and Cardiovascular Medicine
Subjects
Details
- ISSN :
- 00097330
- Volume :
- 45
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Circulation research
- Accession number :
- edsair.doi.dedup.....d66fdb0168e11a7901c649a74cab8c02