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miR-451a Inhibited Cell Proliferation and Enhanced Tamoxifen Sensitive in Breast Cancer via Macrophage Migration Inhibitory Factor
- Source :
- BioMed Research International, Vol 2015 (2015), BioMed Research International
- Publication Year :
- 2015
- Publisher :
- Hindawi Publishing Corporation, 2015.
-
Abstract
- This study aims to investigate the regulative effects of microRNA-451a (miR-451a) on cell proliferation and sensitivity to tamoxifen in breast cancer cells. In cell culture experiments, the lentiviral vectors of pHBLV-miR-451a and pHBLV-miR-451a sponge were constructed and used to transfect MCF-7 and LCC2 cells. The transfection efficiency was tested by fluorescent observation, and cell lines with stable over- or downregulated expression of miR-451a were established. The expression of miR-451a and the target gene macrophage migration inhibitory factor (MIF) were detected by real-time reverse transcriptase polymerase chain reaction and/or western blot. Moreover, MTT assay, colony formation, and Transwell invasion assays were also performed. Data showed that the recombinant lentiviral vectors were constructed correctly, and the virus titer was 1 × 108 CFU/mL. The stable transfected cells were obtained. Overexpression of miR-451a downregulated MIF expression in mRNA and protein levels and inhibited cell proliferation, colony formation, and invasion of breast cancer cells. Downregulation of miR-451a upregulated MIF expression and increased breast cancer cell growth, invasion, and tamoxifen sensitivity. In summary, the miR-451a/MIF pathway may play important roles in the biological properties of breast cancer cells and may be a potential therapeutic target for breast cancer.
- Subjects :
- Article Subject
Cell Survival
Molecular Sequence Data
lcsh:Medicine
Breast Neoplasms
Biology
Real-Time Polymerase Chain Reaction
Transfection
General Biochemistry, Genetics and Molecular Biology
Transduction, Genetic
Cell Line, Tumor
medicine
Humans
MTT assay
Neoplasm Invasiveness
Macrophage Migration-Inhibitory Factors
Cell Proliferation
General Immunology and Microbiology
Base Sequence
Cell growth
Sequence Analysis, RNA
Virus Assembly
HEK 293 cells
lcsh:R
Lentivirus
General Medicine
Molecular biology
Gene Expression Regulation, Neoplastic
Intramolecular Oxidoreductases
MicroRNAs
Tamoxifen
Real-time polymerase chain reaction
HEK293 Cells
Cell culture
Macrophage migration inhibitory factor
Female
Research Article
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....d63a8c2f024d8c6e0440abea3d2ac2ac
- Full Text :
- https://doi.org/10.1155/2015/207684