Back to Search Start Over

Guidance-Cue Control of Horizontal Cell Morphology, Lamination, and Synapse Formation in the Mammalian Outer Retina

Authors :
King Wai Yau
Neal S. Peachey
Kim T. Nguyen-Ba-Charvet
Ryota L. Matsuoka
Lu O. Sun
Ivy S. Samuels
Zheng Jiang
Alain Chédotal
Alex L. Kolodkin
Source :
The Journal of Neuroscience. 32:6859-6868
Publication Year :
2012
Publisher :
Society for Neuroscience, 2012.

Abstract

In the vertebrate retina, neuronal circuitry required for visual perception is organized within specific laminae. Photoreceptors convey external visual information to bipolar and horizontal cells at triad ribbon synapses established within the outer plexiform layer (OPL), initiating retinal visual processing. However, the molecular mechanisms that organize these three classes of neuronal processes within the OPL, thereby ensuring appropriate ribbon synapse formation, remain largely unknown. Here we show that mice with null mutations inSema6AorPlexinA4(PlexA4) exhibit a pronounced defect in OPL stratification of horizontal cell axons without any apparent deficits in bipolar cell dendrite or photoreceptor axon targeting. Furthermore, these mutant horizontal cells exhibit aberrant dendritic arborization and reduced dendritic self-avoidance within the OPL. Ultrastructural analysis shows that the horizontal cell contribution to rod ribbon synapse formation inPlexA4−/−retinas is disrupted. These findings define molecular components required for outer retina lamination and ribbon synapse formation.

Details

ISSN :
15292401 and 02706474
Volume :
32
Database :
OpenAIRE
Journal :
The Journal of Neuroscience
Accession number :
edsair.doi.dedup.....d6283e632b689b76f7ef9f2be94549d8
Full Text :
https://doi.org/10.1523/jneurosci.0267-12.2012