Back to Search
Start Over
Dissolving Microneedles for Intradermal Vaccination against Shigellosis
- Source :
- Vaccines, Vol 7, Iss 4, p 159 (2019), Pastor, Y, Larrañeta, E, Erhard, Á, Quincooces, G, Peñuelas, I, Irache, J M, Donnelly, R & Gamazo, C 2019, ' Dissolving Microneedles for Intradermal Vaccination against Shigellosis ', Vaccines, vol. 7, no. 4 . https://doi.org/10.3390/vaccines7040159, Vaccines, Volume 7, Issue 4, Dadun. Depósito Académico Digital de la Universidad de Navarra, instname
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Intradermal (ID) immunization is of increasing interest due to the easy accessibility and excellent immunogenic properties of the skin. Among ID immunization methods, dissolving microneedles (MNs) have appeared as an alternative to traditional hypodermic immunization, offering many advantages, such as being an easily administered method, with no need for health personnel, painless, and avoiding the use of needles and sharp wastage. In this study, an affordable and easy-to-produce MNs method was developed based on aqueous blends of 30% w/w poly (methyl vinyl ether-co-maleic anhydride). As an antigen model, a subunit vaccine candidate based on outer membrane vesicles from Shigella flexneri was used. Both unloaded and antigen-loaded MNs were synthetized and characterized. The MNs were successfully validated in an in vitro Parafilm M&reg<br />skin model and in a pig skin ex vivo model. Biodistribution studies were performed in BALB/c mice using 99mTcO4- radiolabeled samples. Results indicated that the vesicle vaccine was successfully released from the MNs and targeted gastrointestinal tract after 6 h post-administration. In vivo immunization and protection studies were performed in BALB/c mice. Mice were intradermally immunized through ear skin with one single dose of 200 g antigenic complex, eliciting the production of specific systemic IgG and mucosal IgA. Moreover, MNs were able to protect mice from an experimental infection with 1&times<br />106 CFU/mouse of S. flexneri four weeks after immunization. This work demonstrates for the first time the potential of outer membrane vesicle-loaded dissolving MNs for ID vaccination against enteropathogens like Shigella.
- Subjects :
- 0301 basic medicine
Biodistribution
Immunology
lcsh:Medicine
Outer membrane vesicles
02 engineering and technology
Article
Microbiology
03 medical and health sciences
Shigella flexneri
SDG 3 - Good Health and Well-being
Antigen
In vivo
Intradermal
vaccine
Drug Discovery
Dissolving microneedles
Medicine
Pharmacology (medical)
shigella
intradermal
Pharmacology
biology
business.industry
lcsh:R
021001 nanoscience & nanotechnology
biology.organism_classification
In vitro
3. Good health
Vaccination
030104 developmental biology
Infectious Diseases
Immunization
dissolving microneedles
Shigella
0210 nano-technology
business
Vaccine
Ex vivo
outer membrane vesicles
Subjects
Details
- Language :
- English
- Volume :
- 7
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Vaccines
- Accession number :
- edsair.doi.dedup.....d6150fc380a0c4ac77ccdd61b6b14361