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Synchrotron radiation circular dichroism spectroscopy: vacuum ultraviolet irradiation does not damage protein integrity
- Source :
- Journal of Synchrotron Radiation. 8:1027-1029
- Publication Year :
- 2001
- Publisher :
- International Union of Crystallography (IUCr), 2001.
-
Abstract
- Synchrotron radiation circular dichroism (SRCD) spectroscopy is an emerging technique for sensitive determination of protein secondary structures and for monitoring of conformational changes. An important issue for its adoption as a useful technique is whether the high-intensity low-wavelength vacuum ultraviolet radiation in the SRCD chemically damages proteins. In this paper, using horse myoglobin as a test sample, it is shown that extensive irradiation in the SRCD does not produce any change in the chemical nature of the protein as detected by either SDS gel electrophoresis or mass spectrometry. In addition, no changes in the protein secondary structure are detectable from the SRCD spectra after extensive exposure to the SRCD beam.
- Subjects :
- Nuclear and High Energy Physics
Circular dichroism
Radiation
Ultraviolet Rays
Circular Dichroism
Analytical chemistry
Proteins
Mass spectrometry
Protein Structure, Secondary
chemistry.chemical_compound
Myoglobin
chemistry
Biophysics
sense organs
Irradiation
Spectroscopy
Instrumentation
Protein secondary structure
Polyacrylamide gel electrophoresis
Synchrotrons
Subjects
Details
- ISSN :
- 09090495
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of Synchrotron Radiation
- Accession number :
- edsair.doi.dedup.....d5d9d31f06f6715beaf926924d0b986a