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Development of novel biocompatible thermosensitive anti-adhesive agents using human-derived acellular dermal matrix
- Source :
- PLoS ONE, Vol 14, Iss 2, p e0212583 (2019), PLoS ONE
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- Postoperative adhesion is a natural phenomenon that occurs in damaged tissue cells. Several anti-adhesion agents are currently used, but there is no leading-edge product with excellent adhesion-preventive effects. The purpose of this study was to develop ideal anti-adhesive agents using human-derived acellular dermal matrix (ADM). We developed 5 new biocompatible thermosensitive anti-adhesion barriers (AABs) using micronized human-derived ADM, hyaluronic acid, and temperature-sensitive and biocompatible synthesized polymers. The biocompatibility, anti-adhesion effect, and biodegradability of these AABs were compared with those of commercial thermosensitive anti-adhesion agents. No cytotoxic effects were observed in vitro and in vivo. Animal testing of adhesion resistance confirmed that the adhesion area, strength, and grade of AAB03 were statistically superior to those of the control group. Factors related to adhesion formation, such as lymphocytes, macrophages, microvessels, and collagen fiber density, were observed using specific staining methods; the results confirmed that AAB03 group exhibited significantly lower macrophage counts, microvessel density, and collagen fiber density than the control groups. Furthermore, AAB03 was completely absorbed by 6 weeks. Thus, AAB03 has the potential to be used as a high-performance anti-adhesion agent.
- Subjects :
- Male
Medical Implants
Biocompatible Materials
Tissue Adhesions
02 engineering and technology
Biochemistry
Physical Chemistry
Rats, Sprague-Dawley
chemistry.chemical_compound
Mice
White Blood Cells
0302 clinical medicine
Animal Cells
Materials Physics
Hyaluronic acid
Materials Testing
Medicine and Health Sciences
Cytotoxic T cell
Macrophage
Multidisciplinary
Viscosity
Physics
Adhesion
Dermis
021001 nanoscience & nanotechnology
Extracellular Matrix
Chemistry
030220 oncology & carcinogenesis
Physical Sciences
Engineering and Technology
Medicine
Cellular Types
0210 nano-technology
Immunohistochemical Analysis
Research Article
Biotechnology
Biocompatibility
Immune Cells
Science
Immunology
Materials Science
Surgical and Invasive Medical Procedures
Bioengineering
Minimally Invasive Surgery
Research and Analysis Methods
Cell Line
03 medical and health sciences
In vivo
Animals
Humans
Immunohistochemistry Techniques
Blood Cells
Macrophages
Biology and Life Sciences
Proteins
Cell Biology
In vitro
Rats
Histochemistry and Cytochemistry Techniques
chemistry
Chemical Properties
Cell culture
Immunologic Techniques
Laparoscopy
Medical Devices and Equipment
Collagens
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....d5cd16c6715a760deb5282e4abf73e6a