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Genetic Context and Structural Diversity of Class 1 Integrons from Human Commensal Bacteria in a Hospital Intensive Care Unit▿†
- Publication Year :
- 2011
- Publisher :
- American Society for Microbiology, 2011.
-
Abstract
- Most surveys for class 1 integrons are at least partly predicated on PCR screening that targets integron conserved regions. However, class 1 integrons are structurally diverse, so dependence on conserved regions may lead to missing clinically relevant examples of class 1 integrons. Here, we surveyed a commensal population of bacteria from patients in an intensive care unit to identify class 1 integrons irrespective of their structure or genetic context. We identified several examples of class 1 integrons linked to complete Tn 402 -like or Tn 402 hybrid transposition modules and diverse insertion points with respect to the inverted repeat IRi boundary. The diversity and abundance of class 1 integrons identified are such that many novel elements seen here would not have been identified by commonly used methods, and they revealed an additional level of complexity.
- Subjects :
- DNA, Bacterial
Population
Molecular Sequence Data
Context (language use)
Integron
Microbiology
Polymerase Chain Reaction
Bacterial genetics
law.invention
Epidemiology and Surveillance
Integrons
Transposition (music)
Enterobacteriaceae
law
Drug Resistance, Bacterial
Gram-Negative Bacteria
Humans
Pharmacology (medical)
education
Pharmacology
Genetics
education.field_of_study
biology
Inverted Repeat Sequences
Sequence Analysis, DNA
biochemical phenomena, metabolism, and nutrition
Commensalism
bacterial infections and mycoses
Class (biology)
Intensive care unit
Intensive Care Units
Klebsiella pneumoniae
Infectious Diseases
biology.protein
DNA Transposable Elements
bacteria
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....d5c7ceeac2c86e203d326fbb55fb0504