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AMPAR/TARP stoichiometry differentially modulates channel properties
- Source :
- eLife, Dipòsit Digital de la UB, Universidad de Barcelona, eLife, Vol 9 (2020)
- Publication Year :
- 2019
-
Abstract
- SummaryAMPARs control fast synaptic communication between neurons and their function relies on auxiliary subunits, which importantly modulate channel properties. Although it has been suggested that AMPARs can bind to TARPs with variable stoichiometry, little is known about the effect that this stoichiometry exerts on certain AMPAR properties. Here we have found that AMPARs show a clear stoichiometry dependent modulation although AMPARs still need to be fully saturated with TARPs to show some typical TARP-induced characteristics (i.e. an increase in channel conductance). We also have uncovered important differences in the stoichiometric modulation between calcium-permeable and calcium-impermeable AMPARs. Moreover, in heteromeric AMPARs, TARP positioning in the complex is important to exert certain TARP-dependent features. Finally, by comparing data from recombinant receptors with endogenous AMPAR currents from cerebellar granule cells, we have determined a likely functional stoichiometry of 2 TARPs associated with GluA2 subunits in the somatic AMPARs found in this cell type.
- Subjects :
- 0301 basic medicine
Cell type
Patch-Clamp Techniques
Mouse
QH301-705.5
Science
Primary Cell Culture
Endogeny
AMPA receptor
AMPAR
TARP
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Cerebellum
Animals
Humans
Biology (General)
Receptor
Electrofisiologia
Neural transmission
General Immunology and Microbiology
Chemistry
General Neuroscience
musculoskeletal, neural, and ocular physiology
General Medicine
out-side out
stoichiometry
Electrophysiology
Mice, Inbred C57BL
030104 developmental biology
HEK293 Cells
nervous system
Receptors, Glutamate
cerebellar granule cells
Biophysics
Medicine
Neurotransmissió
channel conductance
Calcium Channels
030217 neurology & neurosurgery
Stoichiometry
Research Article
Neuroscience
Human
Subjects
Details
- ISSN :
- 2050084X
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....d5c5c1df50c0fec41019a6cf5b4cb264