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Deletion of Many Yeast Introns Reveals a Minority of Genes that Require Splicing for Function
- Source :
- Molecular Biology of the Cell. 19:1932-1941
- Publication Year :
- 2008
- Publisher :
- American Society for Cell Biology (ASCB), 2008.
-
Abstract
- Splicing regulates gene expression and contributes to proteomic diversity in higher eukaryotes. However, in yeast only 283 of the 6000 genes contain introns and their impact on cell function is not clear. To assess the contribution of introns to cell function, we initiated large-scale intron deletions in yeast with the ultimate goal of creating an intron-free model eukaryote. We show that about one-third of yeast introns are not essential for growth. Only three intron deletions caused severe growth defects, but normal growth was restored in all cases by expressing the intronless mRNA from a heterologous promoter. Twenty percent of the intron deletions caused minor phenotypes under different growth conditions. Strikingly, the combined deletion of all introns from the 15 cytoskeleton-related genes did not affect growth or strain fitness. Together, our results show that although the presence of introns may optimize gene expression and provide benefit under stress, a majority of introns could be removed with minor consequences on growth under laboratory conditions, supporting the view that many introns could be phased out of Saccharomyces cerevisiae without blocking cell growth.
- Subjects :
- Saccharomyces cerevisiae Proteins
RNA Splicing
Genes, Fungal
Saccharomyces cerevisiae
Biology
Splicing factor
Exon
Minor spliceosome
Gene Expression Regulation, Fungal
Group I catalytic intron
Selection, Genetic
Deoxyribonucleases, Type II Site-Specific
Promoter Regions, Genetic
Molecular Biology
Gene
Cytoskeleton
Sequence Deletion
Genetics
Gene Expression Profiling
Intron
Articles
Cell Biology
Group II intron
Introns
Phenotype
RNA splicing
Subjects
Details
- ISSN :
- 19394586 and 10591524
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....d5b5c1d75a37b155fa718dd2b099820e
- Full Text :
- https://doi.org/10.1091/mbc.e07-12-1254