Back to Search
Start Over
Thinner inhalation effects on oxidative stress and DNA repair in a rat model of abuse
- Source :
- Journal of Applied Toxicology.
- Publication Year :
- 2009
- Publisher :
- Wiley, 2009.
-
Abstract
- Humans can come into contact with thinner by occupational exposure or by intentional inhalation abuse. Numerous studies of workers for genotoxic effects of thinner exposure have yielded conflicting results, perhaps because co-exposure to variable other compounds cannot be avoided in workplace exposure studies. In contrast, there is no data concerning the genotoxic effects of intentional inhalation abuse. The aim of this project was to examine the genotoxic effects of thinner inhalation in an animal model of thinner abuse (rats exposed to 3000 ppm toluene, a high solvent concentration over a very short, 15 min time period, twice a day for 6 weeks). The data presented here provides evidence that thinner inhalation in our experimental conditions is able to induce weight loss, lung abnormalities and oxidative stress. This oxidative stress induces oxidative DNA damage that is not a characteristic feature of genotoxic damage. No significant difference in DNA damage and DNA repair (biomarkers of genotoxicity) in lymphocytes from thinner-treated and control rats was found. Lead treatment was used as a positive control in these assays. Finally, bone marrow was evaluated as a biomarker of cellular alteration associated with thinner inhalation. The observed absence of hemopoietic and genetic toxicity could be explained in part by the absence of benzene, the only carcinogenic component of thinner; however, benzene is no longer a common component of thinner. In conclusion, thinner did not cause genotoxic effects in an experimental model of intentional abuse despite the fact that thinner inhalation induces oxidative stress.
- Subjects :
- Lung Diseases
Male
medicine.medical_specialty
Time Factors
DNA Repair
Substance-Related Disorders
DNA damage
DNA repair
Pharmacology
Toxicology
medicine.disease_cause
Rats, Sprague-Dawley
Bone Marrow
Malondialdehyde
medicine
Animals
Lymphocytes
Lung
Carcinogen
Inhalation Exposure
Toluene toxicity
Inhalation
Chemistry
Body Weight
Rats
Surgery
Disease Models, Animal
Oxidative Stress
Toxicity
Solvents
sense organs
Oxidation-Reduction
Biomarkers
Oxidative stress
Genotoxicity
DNA Damage
Mutagens
Toluene
Subjects
Details
- ISSN :
- 10991263 and 0260437X
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Toxicology
- Accession number :
- edsair.doi.dedup.....d5a732f2fb6e116936449b1b92f9c4a4