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Extraction of koumine from Gelsemium Elegans Benth. and its therapeutic effect on collagen-induced arthritis in mice

Authors :
Ren Zhang
Yao Kuang
Zhengfei Li
Jieying Zhang
Source :
Food Science and Technology (2021), Food Science and Technology, Issue: ahead, Published: 24 MAY 2021, Food Science and Technology v.42 2022, Food Science and Technology (Campinas), Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA), instacron:SBCTA, Food Science and Technology, Volume: 42, Article number: e10421, Published: 24 MAY 2021
Publication Year :
2022
Publisher :
FapUNIFESP (SciELO), 2022.

Abstract

This study aimed to investigate the therapeutic effect of koumine on collagen-induced arthritis (CIA) in mice. Koumine was extracted from Gelsemium Elegans Benth. The CIA model was established in Balb/c mice. Forty successfully modeled mice were randomly divided into model group and 2, 4 and 8 mg/kg koumine groups, 10 mice in each group. Another 10 normal mice were selected as control group. The 2, 4 and 8 mg/kg koumine groups were treated with 2, 4 and 8 mg/kg koumine, respectively, for three successive weeks. At the end of treatment, compared with model group, in 4 and 8 mg/kg koumine groups the arthritis index was significantly decreased (P < 0.05), the peripheral blood interleukin-17 level and T helper 17 (Th17) cell percentage were significantly decreased (P < 0.05), the peripheral blood interleukin-10 level and regulatory T (Treg) cell percentage were significantly increased (P < 0.05), the spleen tissue RORγt protein expression level was significantly decreased (P < 0.05), and the spleen tissue Foxp3 protein expression level was significantly increased (P < 0.05). In conclusion, koumine has therapeutic effect on CIA in mice. The mechanism may be related to its regulating the RORγt/Foxp3 expressions, thus correcting the Th17/Treg immune imbalance.

Details

ISSN :
1678457X and 01012061
Volume :
42
Database :
OpenAIRE
Journal :
Food Science and Technology
Accession number :
edsair.doi.dedup.....d59b08c46000bbe057d5dcb72e57c4cd
Full Text :
https://doi.org/10.1590/fst.10421