Back to Search
Start Over
Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures
- Source :
- Nutrients, Vol 10, Iss 9, p 1305 (2018), Nutrients, Volume 10, Issue 9
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- Epidemiological studies show that fruit consumption may modulate bone mineral density. However, data regarding the effect of the Citrus bergamia Risso (Bergamot orange), a citrus fruit containing a high concentration of flavonoids, on bone health are still lacking. In this study, we investigated the effects of Bergamot polyphenols on the Wnt/&beta<br />catenin pathway in two distinct bone cell types (Saos-2 and MG63). Findings showed that exposure to 0.01 and 0.1 mg/mL doses upregulate &beta<br />catenin expression (p = 0.001), osteoblast differentiation markers (e.g., RUNX2 and COL1A), and downregulate RANKL (p = 0.028), as compared to the control. Our results highlight, for the first time, that Bergamot polyphenols act on bone cells through the &beta<br />catenin pathway. In vivo studies are necessary to fully understand Bergamot&rsquo<br />s role against bone resorption.
- Subjects :
- 0301 basic medicine
Citrus
Saos-2
Core Binding Factor Alpha 1 Subunit
citrus fruits
Pharmacology
Bergamot orange
0302 clinical medicine
Bone cell
Phosphorylation
Wnt Signaling Pathway
beta Catenin
Bone mineral
Mitogen-Activated Protein Kinase 1
Nutrition and Dietetics
Mitogen-Activated Protein Kinase 3
biology
Chemistry
Wnt signaling pathway
bone cells
food and beverages
Osteoblast
Cell Differentiation
medicine.anatomical_structure
RANKL
Bone Remodeling
lcsh:Nutrition. Foods and food supply
Bergamot
030209 endocrinology & metabolism
lcsh:TX341-641
Bone resorption
Article
Collagen Type I
Cell Line
03 medical and health sciences
medicine
Humans
polyphenols
Osteoblasts
Plant Extracts
RANK Ligand
β-catenin
osteoporosis
Enzyme Activation
030104 developmental biology
Fruit
Citrus bergamia
biology.protein
business
business.product_ingredient
Biomarkers
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Volume :
- 10
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....d5946db445dabada1bce0fad1fa6e8bf