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Phrenic Long-Term Facilitation Requires PKCθ Activity within Phrenic Motor Neurons
- Publication Year :
- 2015
- Publisher :
- Society for Neuroscience, 2015.
-
Abstract
- Acute intermittent hypoxia (AIH) induces a form of spinal motor plasticity known as phrenic long-term facilitation (pLTF); pLTF is a prolonged increase in phrenic motor output after AIH has ended. In anesthetized rats, we demonstrate that pLTF requires activity of the novel PKC isoform, PKCθ, and that the relevant PKCθ is within phrenic motor neurons. Whereas spinal PKCθ inhibitors block pLTF, inhibitors targeting other PKC isoforms do not. PKCθ is highly expressed in phrenic motor neurons, and PKCθ knockdown with intrapleural siRNAs abolishes pLTF. Intrapleural siRNAs targeting PKCζ, an atypical PKC isoform expressed in phrenic motor neurons that underlies a distinct form of phrenic motor plasticity, does not affect pLTF. Thus, PKCθ plays a critical role in spinal AIH-induced respiratory motor plasticity, and the relevant PKCθ is localized within phrenic motor neurons. Intrapleural siRNA delivery has considerable potential as a therapeutic tool to selectively manipulate plasticity in vital respiratory motor neurons.
- Subjects :
- Male
Small interfering RNA
Long-Term Potentiation
Rats, Sprague-Dawley
Medicine
Animals
Hypoxia
Protein Kinase Inhibitors
Protein kinase C
Protein Kinase C
Phrenic nerve
Motor Neurons
business.industry
General Neuroscience
Long-term potentiation
Intermittent hypoxia
Articles
Motor neuron
Hypoxia (medical)
Spinal cord
musculoskeletal system
Rats
Enzyme Activation
Isoenzymes
Phrenic Nerve
medicine.anatomical_structure
nervous system
Protein Kinase C-theta
medicine.symptom
business
Neuroscience
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....d58dd4ee8e08ce6d137aa149e38a2209