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RIPK3-Mediated Necroptosis in Diabetic Cardiomyopathy Requires CaMKII Activation

Authors :
Yuyun Hua
Yue Ding
Xinshuai Li
Guoliang Meng
Yun Chen
Wei Zhang
Source :
Oxidative Medicine and Cellular Longevity, Oxidative Medicine and Cellular Longevity, Vol 2021 (2021)
Publication Year :
2021
Publisher :
Hindawi, 2021.

Abstract

Activation of Ca2+/calmodulin-dependent protein kinase (CaMKII) has been proved to play a vital role in cardiovascular diseases. Receptor-interaction protein kinase 3- (RIPK3-) mediated necroptosis has crucially participated in cardiac dysfunction. The study is aimed at investigating the effect as well as the mechanism of CaMKII activation and necroptosis on diabetic cardiomyopathy (DCM). Wild-type (WT) and the RIPK3 gene knockout (RIPK3-/-) mice were intraperitoneally injected with 60 mg/kg/d streptozotocin (STZ) for 5 consecutive days. After 12 w of feeding, 100 μL recombinant adenovirus solution carrying inhibitor 1 of protein phosphatase 1 (I1PP1) gene was injected into the caudal vein of mice. Echocardiography, myocardial injury, CaMKII activity, necroptosis, RIPK1 expression, mixed lineage kinase domain-like protein (MLKL) phosphorylation, and mitochondrial ultrastructure were measured. The results showed that cardiac dysfunction, CaMKII activation, and necroptosis were aggravated in streptozotocin- (STZ-) stimulated mice, as well as in (Lepr) KO/KO (db/db) mice. RIPK3 deficiency alleviated cardiac dysfunction, CaMKII activation, and necroptosis in DCM. Furthermore, I1PP1 overexpression reversed cardiac dysfunction, myocardial injury and necroptosis augment, and CaMKII activity enhancement in WT mice with DCM but not in RIPK3-/- mice with DCM. The present study demonstrated that CaMKII activation and necroptosis augment in DCM via a RIPK3-dependent manner, which may provide therapeutic strategies for DCM.

Details

Language :
English
ISSN :
19420994 and 19420900
Volume :
2021
Database :
OpenAIRE
Journal :
Oxidative Medicine and Cellular Longevity
Accession number :
edsair.doi.dedup.....d5868a1fb7e6b0eb8604060c6cc292b4