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lncRNA TPTEP1 inhibits stemness and radioresistance of glioma through miR‑106a‑5p‑mediated P38 MAPK signaling
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2020
- Publisher :
- Spandidos Publications, 2020.
-
Abstract
- Glioma is diagnosed as the most common intracranial malignant tumor. Cancer stem cells determine stemness and radioresistance, and may facilitate glioma recurrence. The present study aimed to investigate whether the long non-coding RNA (lncRNA) transmembrane phosphatase with tensin homology pseudogene 1 (TPTEP1) regulated cell stemness and radioresistance of glioma, and determine the underlying molecular mechanism of TPTEP1 in the modulation of glioma progression. Cell and molecular biology techniques were applied for investigating the role of TPTEP1 in glioma cell lines, animal model, and clinical samples. The results demonstrated that TPTEP1 attenuated stemness and radioresistance of glioma both in vitro and in vivo. In addition, TPTEP1 augmented MAPK14 expression by competitively interacting with microRNA (miR)-106a-5p, thus activating the P38 MAPK signaling pathway, and suppressing glioma stemness and radioresistance. TPTEP1 functionally bound to miR-106a-5p, which formed a reciprocal regulatory loop to stimulate the P38 MAPK signaling pathway. Low TPTEP1 expression levels were detected in high-grade glioma tissues compared with low-grade glioma tissues, and were positively associated with poor prognosis of patients with glioma. Furthermore, analysis using data from The Cancer Genome Atlas database confirmed the molecular mechanism and biological significance of dysregulation of TPTEP1 in glioma progression. Taken together, the results of the present study suggest that TPTEP1 may be applied as a diagnostic and prognostic indicator for glioma, and may be an alternative target for the treatment of glioma.
- Subjects :
- 0301 basic medicine
Cancer Research
Cell
Apoptosis
Radiation Tolerance
p38 Mitogen-Activated Protein Kinases
Biochemistry
MAPK14
0302 clinical medicine
glioma
Brain Neoplasms
Articles
Cell cycle
Prognosis
Gene Expression Regulation, Neoplastic
microRNA-106a-5p
radioresistance
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Disease Progression
Neoplastic Stem Cells
Molecular Medicine
RNA, Long Noncoding
Pseudogenes
Signal Transduction
MAP Kinase Signaling System
Biology
stemness
03 medical and health sciences
Cancer stem cell
Cell Line, Tumor
Glioma
Radioresistance
microRNA
Genetics
medicine
Humans
neoplasms
Molecular Biology
Cell Proliferation
Oncogene
PTEN Phosphohydrolase
Membrane Proteins
medicine.disease
Molecular medicine
nervous system diseases
MicroRNAs
transmembrane phosphatase with tensin homology pseudogene 1
030104 developmental biology
Cancer research
Neoplasm Recurrence, Local
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....d564769fc7e5740fce268e748c04dea0