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Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters
- Source :
- Toxicology Reports, Toxicology Reports, Vol 8, Iss, Pp 1948-1954 (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Graphical abstract<br />Highlights • Co-exposure to heat and psychological stressors on semen quality have been studied. • Combined exposure group had significantly lower semen quality compared with those of others. • Heat exposure group had a higher percentage of sperm DNA damage compared to others.<br />The present study aims to investigate the effects of co-exposure to heat and psychological stress on sperm DNA and semen parameters among male rats. The study was conducted on 40 healthy adult male Wistar rats. The rats were randomly categorized into four groups of same size consisting of a control group, a heat stress, psychological and co-exposure groups. The heat stress group was exposed to a temperature of 36 °C at 20% relative humidity. The psychological stress exposure group was subjected to three stressors including exposure to strobe light, noise and tilting cage. According the results,the co-exposure group had lower mean sperm parameters including sperm count (17.22 ± 4.22 106/ml), motility (42.63 ± 12.95 %), viability (48.50 ± 23.25 %), normal morphology (56 ± 7.5%), progressive motility (11.61 ± 7.81%), non-progressive motility (31.18 ± 7.77%), curvilinear velocity (24.11 ± 3.81 μm/s) and straight-line velocity (3.2 ± 1.4 μm/s) when compared with those of the other groups (P = 0.001). Mean sperm immobility (57.36 ± 12.95%) and non-progressive motility (37.93 ± 11.15%) in the co-exposure group was higher compared to the other groups (P = 0.001 and P = 0.333, respectively). Assessment of damage to sperm DNA revealed that the heat exposure group had a higher percentage of sperm DNA damage (9.44 ± 6.80 %) compared to others (P = 0.185). In case of all of exposure scenario, the chance that the semen quality decreased compared to the control group has been increased. In general the combined stress had a greater significant effect on sperm parameters compared to other exposure groups, except for DNA damage.
- Subjects :
- Heat Stress
DNA damage
Health, Toxicology and Mutagenesis
Motility
Psychological Stress
Semen
AO, Acridine Orange
Toxicology
Andrology
Semen quality
RA1190-1270
Medicine
THI, Temperature Humidity Index
Co-exposure
CASA, Computer-Assisted Sperm Analysis
ComputingMethodologies_COMPUTERGRAPHICS
business.industry
ANOVA, Analysis OF Variance
Sperm DNA
Stressor
Sperm dna
Regular Article
Sperm
CPCSEA, Committee for the Purpose Of Control and Supervision Of Experiments on Animals
Toxicology. Poisons
Rat
BSA, Bovine Serum Albumin
Co exposure
business
DNA, Deoxyribonucleic Acid
ROS, Reactive Oxygen Species
Subjects
Details
- Language :
- English
- ISSN :
- 22147500
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Toxicology Reports
- Accession number :
- edsair.doi.dedup.....d55425a0eda208f490a771fe2ad38b60