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RNA Interference by Cyanobacterial Feeding Demonstrates the SCSG1 Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in Stentor
- Source :
- Microorganisms, Vol 9, Iss 176, p 176 (2021), Microorganisms, Volume 9, Issue 1
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- In the giant ciliate Stentor coeruleus, oral apparatus (OA) regeneration is an experimentally tractable regeneration paradigm that occurs via a series of morphological steps. OA regeneration is thought to be driven by a complex regulatory system that orchestrates the temporal expression of conserved and specific genes. We previously identified a S. coeruleus-specific gene (named SCSG1) that was significantly upregulated during the ciliogenesis stages of OA regeneration, with an expression peak at the stage of the first OA cilia appearance. We established a novel RNA interference (RNAi) method through cyanobacteria Synechocystis sp. PCC6803 feeding in S. coeruleus. The expression of SCSG1 gene was significantly knocked down by using this method and induced abnormal ciliogenesis of OA regeneration in S. coeruleus, suggesting that SCSG1 is essential for OA regeneration in S. coeruleus. This novel RNAi method by cyanobacterial feeding has potential utility for studying other ciliates.
- Subjects :
- Microbiology (medical)
Oral apparatus
novel RNAi method
ciliate
Microbiology
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
RNA interference
Virology
Ciliogenesis
Gene
lcsh:QH301-705.5
030304 developmental biology
0303 health sciences
biology
Regeneration (biology)
Cilium
Synechocystis sp. PCC6803
biology.organism_classification
Cell biology
nervous system
lcsh:Biology (General)
Stentor coeruleus
030217 neurology & neurosurgery
oral apparatus regeneration
Subjects
Details
- Language :
- English
- ISSN :
- 20762607
- Volume :
- 9
- Issue :
- 176
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....d539d526c8877fd6596b49e7bf89a99c