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Rumen cellulosomics: divergent fiber-degrading strategies revealed by comparative genome-wide analysis of six ruminococcal strains
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2014, 9 (7), pp.e99221. ⟨10.1371/journal.pone.0099221⟩, Plos One 7 (9), e99221. (2014), PLoS ONE, Vol 9, Iss 7, p e99221 (2014), PLoS ONE, 2014, 9 (7), pp.e99221. ⟨10.1371/journal.pone.0099221⟩
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- International audience; Background: A complex community of microorganisms is responsible for efficient plant cell wall digestion by many herbivores, notably the ruminants. Understanding the different fibrolytic mechanisms utilized by these bacteria has been of great interest in agricultural and technological fields, reinforced more recently by current efforts to convert cellulosic biomass to biofuels. Methodology/Principal Findings: Here, we have used a bioinformatics-based approach to explore the cellulosome-related components of six genomes from two of the primary fiber-degrading bacteria in the rumen: Ruminococcus flavefaciens (strains FD-1, 007c and 17) and Ruminococcus albus (strains 7, 8 and SY3). The genomes of two of these strains are reported for the first time herein. The data reveal that the three R. flavefaciens strains encode for an elaborate reservoir of cohesin-and dockerin-containing proteins, whereas the three R. albus strains are cohesin-deficient and encode mainly dockerins and a unique family of cell-anchoring carbohydrate-binding modules (family 37). Conclusions/Significance: Our comparative genome-wide analysis pinpoints rare and novel strain-specific protein architectures and provides an exhaustive profile of their numerous lignocellulose-degrading enzymes. This work provides blueprints of the divergent cellulolytic systems in these two prominent fibrolytic rumen bacterial species, each of which reflects a distinct mechanistic model for efficient degradation of cellulosic biomass.
- Subjects :
- [SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Microorganism
Glycobiology
ruminant
digestion
Genome
Biochemistry
lignocellulose
Ruminococcus
bactérie fibrolytique
Genome Sequencing
0303 health sciences
rumen
Multidisciplinary
Ecology
Genomics
Agricultural sciences
Enzymes
ruminococcus flavefaciens
Cellulosic ethanol
Medicine
dégradation
Digestion
paroi cellulaire
Research Article
Science
Computational biology
Biology
Microbiology
Microbial Ecology
Cell wall
03 medical and health sciences
Rumen
Bacterial Proteins
Genetics
biomasse
Cellulose
Molecular Biology Techniques
Sequencing Techniques
Molecular Biology
030304 developmental biology
030306 microbiology
génome
Ecology and Environmental Sciences
Biology and Life Sciences
Proteins
biology.organism_classification
Enzymology
Biocatalysis
Molecular Complexes
Bacteria
Sciences agricoles
Genome, Bacterial
Genome-Wide Association Study
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Public Library of Science, 2014, 9 (7), pp.e99221. ⟨10.1371/journal.pone.0099221⟩, Plos One 7 (9), e99221. (2014), PLoS ONE, Vol 9, Iss 7, p e99221 (2014), PLoS ONE, 2014, 9 (7), pp.e99221. ⟨10.1371/journal.pone.0099221⟩
- Accession number :
- edsair.doi.dedup.....d5232fb61df42a2c0ff957e6c0829dcf