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Long-chain cationic derivatives of PTA (1,3,5-triaza-7-phosphaadamantane) as new components of potential non-viral vectors
- Source :
- International journal of pharmaceutics. 431(1-2)
- Publication Year :
- 2012
-
Abstract
- The purpose of this study was to investigate the potential of new positively charged solid lipid nanoparticles (SLN) to convey nucleic acids. The cationic character of SLN was obtained by adding as cationic molecules two different long-chain cationic phosphines (CP), namely hexadecyl-PTA iodide (CP16) and octadecyl-PTA iodide (CP18). The obtained CP-SLN are characterized by a positive charge on the surface and reproducible dimensions around 220 nm. These nanosystems are able to efficiently bind nucleic acid molecules and to protect DNA from the activity of serum nucleases up to 120 min. Lastly, in vitro experiments demonstrated that CP-SLN exhibit a quite pronounced antiproliferative effect on cultured human K562 erythroleukemic cells and a limited effect as transfecting adjuvant. These data, and particularly the ability of CP-SLN to protect DNA from degradation, encourages further studies aimed at proposing these nanosystems as a potential approach to deliver nucleic acid to cells in living organisms.
- Subjects :
- non-viral gene delivery systems
Stereochemistry
Surface Properties
Iodide
Genetic Vectors
Pharmaceutical Science
Adamantane
Transfection
Cell Line
chemistry.chemical_compound
Organophosphorus Compounds
Cricetinae
Solid lipid nanoparticle
PTA
cationic solid lipid nanoparticles
transfection
Animals
Humans
Cationic liposome
Particle Size
Triglycerides
Cell Proliferation
chemistry.chemical_classification
Cationic polymerization
DNA
Combinatorial chemistry
In vitro
chemistry
Nucleic acid
Nanoparticles
K562 Cells
Subjects
Details
- ISSN :
- 18733476
- Volume :
- 431
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- International journal of pharmaceutics
- Accession number :
- edsair.doi.dedup.....d51df56d333ea590032b3ed35f0790a1