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Identification of a NCR+/NKG2D+/LFA-1(low)/CD94(-) immature human NK cell subset
- Source :
- Cytometry. Part A : the journal of the International Society for Analytical Cytology. 75(11)
- Publication Year :
- 2009
-
Abstract
- CD56(bright) natural killer (NK) cells, generated in vitro from CD34+ hematopoietic progenitor cells, were characterized after a 30-day culture with flt3 ligand plus IL-15. Virtually, all CD56(bright) cells expressed CD117, CD25, natural cytotoxicity receptors (NCRs), NKG2D, CD161, and CD244, while only a subset expressed CD18-CD11a (LFA-1), and CD94 molecule, defining an immature CD56(bright)/NCRs+/NKG2D+/LFA-1(-)/CD94(-) subset. Another small subset of cells expressing CD94 but not LFA-1 integrin was also identified, suggesting that during NK differentiation LFA-1 might be upregulated later than CD94. To verify this hypothesis in vivo, we evaluated the NK cell expression of LFA-1 in both peripheral and umbilical cord blood samples. Interestingly, in these blood fluids, we have identified a lineage negative CD34(-)/LFA-1(low)/NKp46(dim)/NKG2D(dim)/CD94(-) subset that resembled an immature stage of NK cells present in lymph nodes. Altogether, the results indicate that CD18-CD11a integrin, as well as CD11b in mice, may be a useful marker to identify immature stages of NK cell differentiation.
- Subjects :
- Histology
Time Factors
Cellular differentiation
CD34
chemical and pharmacologic phenomena
Biology
Models, Biological
Pathology and Forensic Medicine
Interleukin 21
Mice
NK-92
Antigens, CD
Signaling Lymphocytic Activation Molecule Family
Cell Adhesion
Animals
Humans
IL-2 receptor
CD11a Antigen
Receptors, Immunologic
Interleukin-15
Lymphokine-activated killer cell
CD11b Antigen
Interleukin-2 Receptor alpha Subunit
Membrane Proteins
hemic and immune systems
Cell Biology
NKG2D
Hematopoietic Stem Cells
Lymphocyte Function-Associated Antigen-1
Cell biology
Killer Cells, Natural
Proto-Oncogene Proteins c-kit
Integrin alpha M
NK Cell Lectin-Like Receptor Subfamily K
NK cell differentiation
CD18 Antigens
biology.protein
NK Cell Lectin-Like Receptor Subfamily D
Subjects
Details
- ISSN :
- 15524930
- Volume :
- 75
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Cytometry. Part A : the journal of the International Society for Analytical Cytology
- Accession number :
- edsair.doi.dedup.....d5150935f5b4da8565339ca9c11abf9d