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Cytotoxic Effect and Induction of Apoptosis in Human Cervical Cancer Cells by Antrodia camphorata
- Source :
- The American Journal of Chinese Medicine. 41:1169-1180
- Publication Year :
- 2013
- Publisher :
- World Scientific Pub Co Pte Lt, 2013.
-
Abstract
- Antrodia camphorata is a Chinese herb indigenous to Taiwan. Previous reports demonstrated that it could induce apoptosis in some cancer cells. The purpose of this study was to investigate the apoptotic effect of the crude extract of A. camphorata in cervical cancer cells. Two human cervical cancer cell lines, HeLa and C-33A, were treated with extract of A. camphorata (10–1000 μg/mL). We found that A. camphorata extract was cytotoxic to both cervical cancer cells in a dose- and time-dependent manner as examined by MTT assay. Treatment with A. camphorata extract at 400 μg/mL induced a 2.3- and 4.4-fold increase in oligonucleosome formation from the cleaved chromosomal DNA in HeLa and C-33A cells, respectively. A. camphorata extract also activated caspase-3, -8, and -9 activities and increased the cytosolic level of cytochrome c in both cell lines as the dosage increased. Furthermore, A. camphorata extract increased expressions of Bak, Bad and Bim, while decreasing expressions of Bcl-2 and Bcl-xL of the Bcl-2 family proteins in HeLa and C-33A cells. The expression of IAP proteins, XIAP and survivin, was also decreased in both cervical cancer cells after treatment with A. camphorata. Our in vitro study suggests that A. camphorata is cytotoxic to cervical cancer cells through both extrinsic and intrinsic apoptotic mechanisms. It could be used as a novel phytotherapeutic agent or auxiliary therapy in the treatment of cervical cancer.
- Subjects :
- Time Factors
Gene Expression
Uterine Cervical Neoplasms
Apoptosis
Biology
HeLa
Proto-Oncogene Proteins
Survivin
Tumor Cells, Cultured
Chromosomes, Human
Humans
Cytotoxic T cell
MTT assay
Molecular Targeted Therapy
Antrodia
Bcl-2-Like Protein 11
Dose-Response Relationship, Drug
Plant Extracts
Cytochromes c
Membrane Proteins
DNA
General Medicine
biology.organism_classification
Nucleosomes
XIAP
bcl-2 Homologous Antagonist-Killer Protein
Proto-Oncogene Proteins c-bcl-2
Complementary and alternative medicine
Biochemistry
Caspases
Cancer cell
Cancer research
Female
bcl-Associated Death Protein
Apoptosis Regulatory Proteins
HeLa Cells
Phytotherapy
Subjects
Details
- ISSN :
- 17936853 and 0192415X
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- The American Journal of Chinese Medicine
- Accession number :
- edsair.doi.dedup.....d5099c0a11e736c253ad70b6d7b7042a