Back to Search
Start Over
Genetic diversity of Candidatus Liberibacter asiaticus based on two hypervariable effector genes in Thailand
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 12, p e112968 (2014)
- Publication Year :
- 2014
-
Abstract
- Huanglongbing (HLB), also known as citrus greening, is one of the most destructive diseases of citrus worldwide. HLB is associated with three species of ‘Candidatus Liberibacter’ with ‘Ca. L. asiaticus’ (Las) being the most widely distributed around the world, and the only species detected in Thailand. To understand the genetic diversity of Las bacteria in Thailand, we evaluated two closely-related effector genes, lasA I and lasA II, found within the Las prophages from 239 infected citrus and 55 infected psyllid samples collected from different provinces in Thailand. The results indicated that most of the Las-infected samples collected from Thailand contained at least one prophage sequence with 48.29% containing prophage 1 (FP1), 63.26% containing prophage 2 (FP2), and 19.38% containing both prophages. Interestingly, FP2 was found to be the predominant population in Las-infected citrus samples while Las-infected psyllids contained primarily FP1. The multiple banding patterns that resulted from amplification of lasA I imply extensive variation exists within the full and partial repeat sequence while the single band from lasA II indicates a low amount of variation within the repeat sequence. Phylogenetic analysis of Las-infected samples from 22 provinces in Thailand suggested that the bacterial pathogen may have been introduced to Thailand from China and the Philippines. This is the first report evaluating the genetic variation of a large population of Ca. L. asiaticus infected samples in Thailand using the two effector genes from Las prophage regions.
- Subjects :
- Citrus
Candidatus Liberibacter
Sequence analysis
Population
Population genetics
lcsh:Medicine
Plant Science
Biology
Microbiology
Microbial Ecology
Hemiptera
Plant Microbiology
Rhizobiaceae
Genetic variation
Genetics
Animals
education
lcsh:Science
Prophage
Phylogeny
Genetic diversity
education.field_of_study
Multidisciplinary
Phylogenetic tree
Ecology
lcsh:R
Genetic Variation
Biology and Life Sciences
Agriculture
Bacteriology
biology.organism_classification
Thailand
Genes, Bacterial
lcsh:Q
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....d4e90e663bf38a251a909c92de32749b