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EPR study of thylakoid membrane dynamics in mutants of the carotenoid biosynthesis pathway of Synechocystis sp. PCC6803
- Source :
- Scopus-Elsevier
- Publication Year :
- 2012
-
Abstract
- EPR spectroscopy using 5-doxylstearic acid (5-SASL) and 16-doxylstearic acid (16-SASL) spin probes was used to study the fluidity of thylakoid membranes. These were isolated from wild type Synechocystis and from several mutants in genes encoding selected enzymes of the carotenoid biosynthesis pathway and/or acyl-lipid desaturases. Cyanobacteria were cultivated at 25°C and 35°C under different light regimes: photoautotrophically (PAG) and/or in light-activated heterotrophic conditions (LAHG). The relative fluidity of membranes was estimated from EPR spectra based on the empirical outermost splitting parameter in a temperature range from 15°C to 40°C. Our findings demonstrate that in native thylakoid membranes the elimination of xanthophylls decreased fluidity in the inner membrane region under optimal growth conditions (25°C) and increased it under sublethal heat stress (35°C). This indicated that the overall fluidity of native photosynthetic membranes in cyanobacteria may be influenced by the ratio of polar to non-polar carotenoid pools under different environmental conditions.
- Subjects :
- chemistry.chemical_classification
Cyanobacteria
biology
Synechocystis
Electron Spin Resonance Spectroscopy
spin labels
biology.organism_classification
Photosynthesis
Carotenoids
Thylakoids
cyanobacteria
General Biochemistry, Genetics and Molecular Biology
Membrane
chemistry
Biochemistry
carotenoid biosynthesis enzymes
lipid desaturases
Xanthophyll
Thylakoid
Inner membrane
Carotenoid
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....d4b9768ff6951bca6c8a625867a264f9